Comparison of the infrapatellar and subcutaneous adipose tissue biopsy material as a source of mesenchymal stromal cells for regenerative medicine in traumatology

Yulia Avramenko, S. Maslennikov, Stanislav Bondarenko, M. Golovakha
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Abstract

Objective. The use of infrapatellar fat pad (IPFP) as the source of mesenchymal stem cells (MSCs), show an age-independent proliferation and differentiation potential. In addition, the pronounced chondrogenic potential of IPFP-ASCs makes them promising candidates for research for use in other methodsof regenerative therapy. Methods. A direct immunohistochemical study was carried out in serial paraffin sections of the biopsy material of Hoffa`s fat pad and subcutaneus fat tissue, using monoclonal antibodies. The minimum criteria established by the International Society for Cell Therapy to ensure the identity of MSCs use CD73 and CD105 as positive markers and CD34, CD31, CD45 as a negative. Results. According to the results of histological, immunohistochemical, morphometric and statistical studies, it was found that in the biopsy of both tissues the relative number of cells with an immunoprofile CD105+, CD73+, CD34–, CD31–, CD45– in the standard field of view (×200) was: 7.25 (5.42; 8.89 ) %, and 11.11 (8.46; 13.45) %, no statistically significant difference was found between comparison groups (р > 0,05). Conclusion. The therapeutic effect of mesenchymal stem cells of subcutaneous adipose tissue has been proven, thus the identification of similar cells in the biopsy material and relatively similar density in the Hoffa body makesit an important source of adipose-derived stem cells that can be used for regenerative engineering tissue.
比较髌下和皮下脂肪组织活检材料作为创伤再生医学间充质基质细胞的来源
目的。使用髌下脂肪垫(IPFP)作为间充质干细胞(MSCs)的来源,显示出与年龄无关的增殖和分化潜力。此外,髌下脂肪垫间质干细胞还具有明显的软骨生成潜能,因此有望用于其他再生治疗方法的研究。研究方法使用单克隆抗体对霍法脂肪垫和皮下脂肪组织活检材料的连续石蜡切片进行了直接免疫组化研究。国际细胞治疗学会制定的确保间充质干细胞身份的最低标准是以 CD73 和 CD105 为阳性标记,CD34、CD31、CD45 为阴性标记。结果根据组织学、免疫组化、形态计量学和统计学研究的结果,发现在两种组织的活检中,标准视野(×200)内具有 CD105+、CD73+、CD34-、CD31-、CD45- 免疫特征的细胞相对数量分别为:7.25(5.42;8.89)% 和 11.11(8.46;13.45)%,比较组之间无统计学差异(р > 0.05)。结论皮下脂肪组织间充质干细胞的治疗效果已得到证实,因此,活检材料中相似细胞的鉴定和霍法体内相对相似的密度使其成为脂肪来源干细胞的重要来源,可用于再生工程组织。
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