Prunus yedoensis Bark Downregulates the Expression of Cell Adhesion Molecules in Human Endothelial Cell Lines and Relaxes Blood Vessels in Rat Aortic Rings

Pharmaceuticals Pub Date : 2024-07-10 DOI:10.3390/ph17070926

Yeoum Choi, Jung-Mo Yang, C. Jeong, Sujin Shin, Junkyu Park, Kyungjin Lee, Ju-Hyun Cho

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Abstract

The incidence of cardiovascular diseases, such as high blood pressure, is increasing worldwide, owing to population aging and irregular lifestyle habits. Previous studies have reported the vasorelaxant effects of Prunus yedoensis bark methanol extract. However, various solvent extracts of P. yedoensis bark and their vascular relaxation mechanisms have not been sufficiently studied. We prepared extracts of P. yedoensis bark using various solvents (water, 30% ethanol, and 70% ethanol). P. yedoensis bark 30% ethanol extract (PYB-30E) decreased the expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin in human umbilical vein endothelial cells (HUVECs) activated with 200 ng/mL TNF-α. Additionally, PYB-30E showed vasodilatory effects on isolated rat aortic rings. This was confirmed to be the result of the activation of the NO/cGMP pathway, regulation of non-selective calcium-activated K+ channels, and calcium channel blockade. Additionally, PYB-30E significantly reduced systolic and diastolic blood pressure in spontaneously hypertensive rats (SHR). Taken together, our results indicated that PYB-30E is a candidate functional material with preventive and therapeutic effects against hypertension.

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杨梅树皮能下调人内皮细胞系中细胞粘附分子的表达并舒张大鼠主动脉环中的血管

由于人口老龄化和不规律的生活习惯，高血压等心血管疾病的发病率在全球范围内不断上升。以前的研究已经报道了Prunus yedoensis 树皮甲醇提取物的血管舒张作用。然而，对P. yedoensis树皮的各种溶剂提取物及其血管舒张机制的研究还不够充分。我们使用不同的溶剂（水、30% 乙醇和 70% 乙醇）制备了崖柏树皮提取物。P. yedoensis 树皮 30% 乙醇提取物（PYB-30E）能降低用 200 ng/mL TNF-α 活化的人脐静脉内皮细胞（HUVECs）中血管细胞粘附分子-1（VCAM-1）、细胞间粘附分子-1（ICAM-1）和 E-选择素的表达。此外，PYB-30E 对离体大鼠主动脉环也有扩张血管的作用。经证实，这是激活 NO/cGMP 通路、调节非选择性钙激活 K+ 通道和阻断钙通道的结果。此外，PYB-30E 还能显著降低自发性高血压大鼠（SHR）的收缩压和舒张压。综上所述，我们的研究结果表明，PYB-30E 是一种具有预防和治疗高血压作用的候选功能材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pharmaceuticals

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