CARBON CYCLE ENZYMES ACTIVITY DYNAMICS UNDER CONDITIONS OF TRANSITION TO MINIMUM PROCESSING TECHNOLOGIES

E. Belousova, A. Belousov
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Abstract

The purpose of research is to study the dynamics of the activity of enzymes in the carbon cycle of agrochernozems and its effect on the transformation of easily mineralizable organic compounds when using waste and surface treatment methods. Field observations were carried out on the basis of the production experience of OOO OPH Dary Malinovki in the Sukhobuzimo District in the Krasnoyarsk forest-steppe (56°10′ N and 91°47′ E). The impact of dump and minimal processing technologies on the seaso¬nal dynamics of the activity of carbon cycle enzymes in the layers of agrochernozem is considered. The field experiment scheme is represented by the following options: moldboard plowing (standard), minimal tillage (surface disking), flat-cut tillage (cultivation). The level of polyphenol oxidase activity in the soil of the experimental variants was estimated to be weak. The intraseasonal dynamics of the studied enzymes was significant and correlated with the transformation of the humus accumulation coefficient. On non-dumping backgrounds, the variability of polyphenol oxidase was less variable. The minimum va¬lues of the humus accumulation coefficient were found at the initial stage of introducing the technologies under study during the fallow period of agrochernozems, as well as during the barley growing season. The maximum is in the soil under spring wheat crops. The use of flat-cut cultivators led to a strong relationship between the activity of polyphenol oxidase and the coefficient of humus accumulation. In the soil of all variants, an inverse relationship was observed between peroxidase activity and the coefficient of humus accumulation. The participation of peroxidase in the biochemical processes of mineralization was carried out mainly under the conditions of dump processing of agrochernozems. The activity of oxidoreductase enzymes indicated favorable conditions for the humification of organic compounds and the accumulation of newly formed humic substances in the soil when spring wheat was placed in fallow under conditions of using no-moldboard technologies.
向最低加工技术过渡条件下的碳循环酶活性动态
研究的目的是研究酶在农作物碳循环中的活性动态,及其在使用废物和表面处理方法时对易矿化有机化合物转化的影响。根据克拉斯诺亚尔斯克森林草原苏霍布齐莫(北纬 56°10′,东经 91°47′)地区 OOO OPH Dary Malinovki 公司的生产经验进行了实地观察。研究考虑了倾倒和最小加工技术对农业沼泽层中碳循环酶活性的海洋动态影响。田间试验方案由以下选项组成:模板耕作(标准)、微耕作(表面耙地)、平切耕作(耕作)。据估计,实验变体土壤中的多酚氧化酶活性水平较弱。所研究的酶的季节内动态变化显著,并与腐殖质积累系数的变化相关。在非倾倒背景下,多酚氧化酶的变化较小。腐殖质累积系数的最小值出现在引入所研究技术的最初阶段,即农业休耕期和大麦生长期。最大值出现在春小麦作物下的土壤中。使用平切耕作机导致多酚氧化酶的活性与腐殖质积累系数之间的密切关系。在所有变种的土壤中,过氧化物酶活性与腐殖质积累系数之间呈反比关系。过氧化物酶参与矿化的生化过程主要是在农作物倾倒加工条件下进行的。氧化还原酶的活性表明,在无模板技术条件下休耕春小麦时,土壤中有机化合物的腐殖化和新形成的腐殖质的积累条件有利。
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