Biotesting of decabromodiphenyl oxide using a multi-component test system

L. P. Erdniev, Yurij S. Gusev, Y. Pleshakova, I. Kosheleva, D. A. Kuzyanov, A. N. Mikerov
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Abstract

   The results of biotesting of decabromodiphenyl oxide using a multicomponent test sys- tem are presented. It has been demonstrated that its aqueous solutions exhibit a reverse dose- dependent effect in stimulating the growth of colonies of the green algae Chlorella vulgaris and show no signs of toxic effects on Daphnia magna. During the thermal decomposition of decabro- modiphenyl oxide at a temperature of 400°C, a mixture of products is formed, one of which is bromine. The decomposition product is released into the air and possesses toxic properties, as evi- denced by stimulation of the growth of colonies of the green algae Chlorella vulgaris, as well as the death and growth abnormalities of Planorbis mollusk embryos. Therefore, water samples con- taining decabromodiphenyl oxide can be assessed for toxicity by biotesting methods, using cul- tures of green algae Chlorella vulgaris and 24-hour-old Planorbis embryos as test subjects. It is noteworthy that in the context of the prevailing perception of the safety of decabromodiphenyl ox- ide, based on its poor water solubility and low toxicity to biological organisms, there arises a need to study embryotoxic effects of the thermal decomposition products on both animals and humans. 
利用多组分测试系统对十溴二苯氧化物进行生物测试
本文介绍了使用多组分测试系统对十溴二苯氧化物进行生物测试的结果。结果表明,十溴二苯氧化物的水溶液在刺激绿藻小球藻菌落生长方面表现出与剂量相关的反向效应,而且对大型蚤没有毒性影响。十溴二苯氧化物在 400°C 的温度下进行热分解时,会产生一种混合物,其中一种是溴。分解产物释放到空气中,具有毒性,具体表现为刺激绿藻 Chlorella vulgaris 群体的生长,以及 Planorbis 软体动物胚胎的死亡和生长异常。因此,含有十溴二苯氧化物的水样可通过生物测试法进行毒性评估,测试对象为绿藻小球藻和 24 小时大的浮游动物胚胎。值得注意的是,由于十溴二苯氧化物的水溶性差,对生物体的毒性低,因此人们普遍认为十溴二苯氧化物是安全的,因此有必要研究热分解产物对动物和人类的胚胎毒性影响。
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