Efficient generation of recombinant anti-HER2 scFv with high yield and purity using a simple method

Abstract

We developed a method to produce a soluble form of a single-chain fragment variable (scFv) targeting human epithelial growth factor receptor 2 (HER2) in Escherichia coli. By optimizing the orientations of the variable heavy (VH) and variable light (VL) domains and the His-tag, we identified the HL-His type antibody with the highest HER2-binding activity. Purification of HL-His yielded 40.7 mg from a 1 L culture, achieving >99% purity. The limit of detection was determined to be 2.9 ng, demonstrating high production yield, purity, and sensitivity. Moreover, we successfully labeled HER2⁺ cell lines with fluorescent dye-conjugated scFv, resulting in a significantly higher observed signal-to-background ratio, compared to that of HER2⁻ cell lines. This highlights the potential of these fluorescent scFvs as valuable probes for HER2⁺ breast cancer diagnostics. Notably, the process for the complete scFv production was streamlined and required only 4–5 days. Additionally, the product maintained its activity after freeze storage, allowing for large-scale production and a wide range of practical applications.