Cannabinoids detected in exhaled breath condensate after cannabis use.

IF 3.7 4区医学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of breath research Pub Date : 2024-07-23 DOI:10.1088/1752-7163/ad6347

Jennifer L Berry, Ashley Brooks-Russell, Cheryle N Beuning, Sarah A Limbacher, Tara M Lovestead, Kavita M Jeerage

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引用次数: 0

Abstract

Cannabinoids can be detected in breath after cannabis use, but different breath matrices need to be explored as studies to date with filter-based devices that collect breath aerosols have not demonstrated that breath-based measurements can reliably identify recent cannabis use. Exhaled breath condensate (EBC) is an unexplored aqueous breath matrix that contains condensed volatile compounds and water vapor in addition to aerosols. EBC was collected from participants both before and at two time points (0.7 ± 0.2 h and 1.7 ± 0.3 h) after observed cannabis use. Eleven different cannabinoids were monitored with liquid chromatography tandem mass spectrometry. Five different cannabinoids, including Δ⁹-tetrahydrocannabinol (THC), were detected in EBC collected from cannabis users. THC was detected in some EBC samples before cannabis use, despite the requested abstinence period. THC was detected in all EBC samples collected at 0.7 h post use and decreased for all participants at 1.7 h. Non-THC cannabinoids were only detected after cannabis use. THC concentrations in EBC samples collected at 0.7 h showed no trend with sample metrics like mass or number of breaths. EBC sampling devices deserve further investigation with respect to modes of cannabis use (e.g, edibles), post use time points, and optimization of cannabinoid recovery.

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吸食大麻后呼出的冷凝物中检测到大麻素。

使用大麻后可在呼气中检测到大麻素，但需要探索不同的呼气基质，因为迄今为止使用基于过滤的设备收集呼气气溶胶的研究并未证明基于呼气的测量能够可靠地识别近期使用大麻的情况。呼出气体冷凝物（EBC）是一种尚未探索的含水呼气基质，除气溶胶外还含有冷凝挥发性化合物和水蒸气。在观察到吸食大麻之前和之后的两个时间点（0.7 h ± 0.2 h 和 1.7 h ± 0.3 h）收集参与者的 EBC。使用液相色谱串联质谱法（LC-MS/MS）监测了 11 种不同的大麻素。从大麻使用者体内收集的 EBC 中检测到五种不同的大麻素，包括 Δ9-四氢大麻酚（THC）。尽管规定了禁食期，但在使用大麻前的一些 EBC 样本中还是检测到了四氢大麻酚。在使用大麻后 0.7 小时采集的所有 EBC 样本中都检测到了四氢大麻酚，而在使用大麻后 1.7 小时，所有参与者的 EBC 样本中检测到的四氢大麻酚浓度都有所下降。在 0.7 小时采集的 EBC 样本中，四氢大麻酚浓度与样本质量或呼吸次数等指标没有趋势关系。EBC 采样设备在大麻使用方式（如食用）、使用后时间点和大麻素回收优化方面值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM

CiteScore

7.60

自引率

21.10%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.