A demonstration that o2− is a crucial intermediate in the high quantum yield luminescence of luminol

E.Ka. Miller, I. Fridovich
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引用次数: 21

Abstract

The chemiluminescence of luminol, due to its reaction with alkaline H2O2, is inhibited by Superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H2O2-induced luminescence of luminol and lessens, but does not eliminate, the sensitivity towards these inhibitors. Reaction mechanisms are proposed to account for these results. Since luminol luminescence depends upon a reaction between the luminol radical and O2, and since the luminol radical can reduce dioxygen to O2, Superoxide dismutase-inhibitable luminol luminescence cannot be reliably used as a detector of O2 production.

证明氧−是鲁米诺高量子产率发光的关键中间体
鲁米诺的化学发光,由于它与碱性H2O2反应,被超氧化物歧化酶或羟基自由基清除剂抑制。血红蛋白显著增强h2o2诱导的鲁米诺发光,降低但不消除对这些抑制剂的敏感性。提出了反应机制来解释这些结果。由于鲁米诺发光依赖于鲁米诺自由基和O2−之间的反应,并且由于鲁米诺自由基可以将二氧还原为O2−,因此超氧化物歧化酶抑制的鲁米诺发光不能可靠地用作O2−产生的检测器。
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