{"title":"A demonstration that o2− is a crucial intermediate in the high quantum yield luminescence of luminol","authors":"E.Ka. Miller, I. Fridovich","doi":"10.1016/S0748-5514(86)80058-7","DOIUrl":null,"url":null,"abstract":"<div><p>The chemiluminescence of luminol, due to its reaction with alkaline H<sub>2</sub>O<sub>2</sub>, is inhibited by Superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H<sub>2</sub>O<sub>2</sub>-induced luminescence of luminol and lessens, but does not eliminate, the sensitivity towards these inhibitors. Reaction mechanisms are proposed to account for these results. Since luminol luminescence depends upon a reaction between the luminol radical and O<sub>2</sub><sup>−</sup>, and since the luminol radical can reduce dioxygen to O<sub>2</sub><sup>−</sup>, Superoxide dismutase-inhibitable luminol luminescence cannot be reliably used as a detector of O<sub>2</sub><sup>−</sup> production.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"2 2","pages":"Pages 107-110"},"PeriodicalIF":0.0000,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0748-5514(86)80058-7","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of free radicals in biology & medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0748551486800587","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
Abstract
The chemiluminescence of luminol, due to its reaction with alkaline H2O2, is inhibited by Superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H2O2-induced luminescence of luminol and lessens, but does not eliminate, the sensitivity towards these inhibitors. Reaction mechanisms are proposed to account for these results. Since luminol luminescence depends upon a reaction between the luminol radical and O2−, and since the luminol radical can reduce dioxygen to O2−, Superoxide dismutase-inhibitable luminol luminescence cannot be reliably used as a detector of O2− production.