Effects of nanoparticles on the activity and resistance genes of anaerobic digestion enzymes in livestock and poultry manure containing the antibiotic tetracycline

IF 2.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Open Chemistry Pub Date : 2024-07-12 DOI:10.1515/chem-2024-0060

Xiaofei Zhen, Ruonan Jiao, Han Zhan, Wenbing Wu, Ke Li, Lei Feng, Tie Du

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Abstract

Taking chicken manure containing antibiotic oxytetracycline (OTC) as the research object, nano-Fe2O3 with a concentration of 300 mg/kg TS and nano-C60 with a concentration of 100 mg/kg TS as carriers were used for a 55-day sequential batch mesophilic anaerobic digestion experiment. The gas production performance, chemical parameters, antibiotic content, enzyme concentration, and resistance gene changes during anaerobic digestion were analyzed to clarify the effect of nanoparticles on anaerobic systems containing antibiotic chicken manure and provide a reliable basis for pollution reduction capacity of livestock and poultry manure. The results showed that (1) adding nano-Fe2O3 and nano-C60 promoted anaerobic gas production in chicken manure with different concentrations of OTC. The cumulative gas production from days 1 to 10 was 2,234(T5) > 2,163(T4) > 1,445(T2) > 1,289(T3) > 1,220(T1) > 1,216(CK) mL. The cumulative gas production of T4 and T5 increased by 77.29 and 83.11%, respectively. The final cumulative gas production for each group was 3,712(CK), 3,993(T1), 4,344(T2), 4,447(T3), 4,671(T4), and 4,849(T5) mL. The final OTC residue concentrations were 15.25, 20.40, 56.56, 17.67, and 16.89 μg/L, with degradation rates of 98.31, 98.80, 98.29, 99.07, and 99.11% respectively; (2) antibiotic OTC increased the activities of dehydrogenase, amylase, protease, and urease, while adding nanoparticles increased the activities of dehydrogenase and amylase, with no significant impact on cellulase, urease, and protease activities; (3) antibiotic resistance gene (ARGs) of various types were found in groups T2, T4, and T5, with the multidrug resistance gene (ARGs)-Multidrug accounting for a high proportion of 33.54, 35.63, and 37.43%, respectively, while the proportions of the other four types MLS, tetracycline, glycopeptide, and peptide ranged from 8.18 to 12.98%.

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纳米颗粒对含有抗生素四环素的畜禽粪便中厌氧消化酶的活性和抗性基因的影响

以含有抗生素土霉素（OTC）的鸡粪为研究对象，以浓度为 300 mg/kg TS 的纳米氧化铁和浓度为 100 mg/kg TS 的纳米 C60 为载体，进行了为期 55 天的序批式中温厌氧消化实验。分析了厌氧消化过程中的产气性能、化学参数、抗生素含量、酶浓度和抗性基因的变化，以明确纳米颗粒对含抗生素鸡粪厌氧系统的影响，为畜禽粪便的污染减排能力提供可靠依据。结果表明：(1) 添加纳米 Fe2O3 和纳米 C60 能促进不同浓度 OTC 鸡粪的厌氧产气。第 1 至 10 天的累计产气量分别为 2 234（T5）毫升；2 163（T4）毫升；1 445（T2）毫升；1 289（T3）毫升；1 220（T1）毫升；1 216（CK）毫升。T4 和 T5 的累计产气量分别增加了 77.29% 和 83.11%。各组的最终累积产气量分别为 3 712（CK）、3 993（T1）、4 344（T2）、4 447（T3）、4 671（T4）和 4 849（T5）毫升。最终的 OTC 残留浓度分别为 15.25、20.40、56.56、17.67 和 16.89 μg/L，降解率分别为 98.31、98.80、98.29、99.07 和 99.11%；（2）抗生素OTC提高了脱氢酶、淀粉酶、蛋白酶和脲酶的活性，而添加纳米颗粒提高了脱氢酶和淀粉酶的活性，对纤维素酶、脲酶和蛋白酶活性无明显影响；（3）T2、T4和T5组中发现了各种类型的抗生素耐药基因（ARGs），其中多药耐药基因（ARGs）-Multidrug占比较高，分别为33.54%、35.63%、37.43%，其他四种类型MLS、四环素、糖肽、多肽所占比例在8.18%~12.98%之间。

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来源期刊

Open Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

6 weeks

期刊介绍： Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. The central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field. The journal is the premier source for cutting edge research in fundamental chemistry and it provides high quality peer review services for its authors across the world. Moreover, it allows for libraries everywhere to avoid subscribing to multiple local publications, and to receive instead all the necessary chemistry research from a single source available to the entire scientific community.

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阿拉丁

Tetracycline