Increase proportion of di-rhamnolipids biosynthesized from Pseudomonas aeruginosa and evaluation of relationship between activity and di-rhamnolipids proportion

IF 1.6 4区 工程技术 Q3 CHEMISTRY, APPLIED
Yuting Wu, Baohang Wang, Yanru Wang, Yitong Yang, Feng Zhao
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Abstract

Rhamnolipids, simply divided into mono-rhamnolipids and di-rhamnolipids, their physicochemical properties are structure-dependent. This study explored to increase the proportion of di-rhamnolipids biosynthesized from Pseudomonas aeruginosa and evaluate the relationship between activity and di-rhamnolipids proportions. P. aeruginosa SGrhlC was constructed by increasing the rhlC gene in P. aeruginosa SG. HPLC-MS results indicated that SGrhlC produced more di-rhamnolipids (62.32%) than that of the wild-type strain SG (45.24%). Both the species and proportion of di-rhamnolipids were increased, mainly Rha-Rha-C8-C10 and Rha-Rha-C10-C10. The rhamnolipids produced by SGrhlC was thermostable and salt-tolerant. The SGrhlC rhamnolipids decreased surface tension of water to 27.0 mN/m with a critical micelle concentration (CMC) of 50 mg/L and emulsified crude oil with an emulsification index of 66.9 ± 1.5%. The SGrhlC rhamnolipids exhibited antimicrobial activity against Staphylococcus aureus and Cladosporium sp. with IC50 (half maximal inhibitory concentration) values less than 15 mg/L, and washed 73.02% ± 1.77% of oil from petroleum-contaminated soil. Physicochemical activities of three bacterial rhamnolipids varied with their di-rhamnolipids proportions. Results indicated that the higher proportions of di-rhamnolipids were, the lower CMC, better surface activity and higher washing oil rate were, while the weaker emulsifying activity and lower antimicrobial activity were. The SGrhlC rhamnolipids showed better surface activity and a lower critical micelle concentration, which was superior for wetting, foaming, desorbing and dispersing. This study revealed that P. aeruginosa can be genetically regulated to biosynthesize rhamnolipids with specific structure. Perspectives of the customized biosynthesis and applications of rhamnolipids were also discussed.

Abstract Image

提高铜绿假单胞菌生物合成的二鼠李糖脂的比例以及评估活性与二鼠李糖脂比例之间的关系
鼠李糖脂简单分为单鼠李糖脂和双鼠李糖脂,其理化性质与结构有关。本研究旨在提高铜绿假单胞菌生物合成的二鼠李糖脂的比例,并评估其活性与二鼠李糖脂比例之间的关系。通过增加铜绿假单胞菌 SG 中的 rhlC 基因,构建了铜绿假单胞菌 SGrhlC。HPLC-MS 结果表明,与野生型菌株 SG(45.24%)相比,SGrhlC 产生了更多的二鼠李糖脂(62.32%)。二鼠李糖脂的种类和比例都有所增加,主要是 Rha-Rha-C8-C10 和 Rha-Rha-C10-C10。SGrhlC 产生的鼠李糖脂具有恒温性和耐盐性。SGrhlC 鼠李糖脂能降低水的表面张力至 27.0 mN/m,临界胶束浓度(CMC)为 50 mg/L,乳化原油的乳化指数为 66.9 ± 1.5%。SGrhlC 鼠李糖脂对金黄色葡萄球菌和克拉多孢霉具有抗菌活性,其 IC50(半最大抑制浓度)值小于 15 毫克/升,并能洗净石油污染土壤中 73.02% ± 1.77% 的油。三种细菌鼠李糖脂的理化活性随其二鼠李糖脂比例的变化而变化。结果表明,二鼠李糖脂比例越高,CMC 越低,表面活性越好,洗油率越高,而乳化活性越弱,抗菌活性越低。SGrhlC 鼠李糖脂显示出更好的表面活性和更低的临界胶束浓度,在润湿、发泡、解吸和分散方面更胜一筹。这项研究揭示了铜绿微囊藻可以通过基因调控来生物合成具有特定结构的鼠李糖脂。研究还探讨了鼠李糖脂的定制生物合成和应用前景。
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来源期刊
Journal of Surfactants and Detergents
Journal of Surfactants and Detergents 工程技术-工程:化工
CiteScore
3.80
自引率
6.20%
发文量
68
审稿时长
4 months
期刊介绍: Journal of Surfactants and Detergents, a journal of the American Oil Chemists’ Society (AOCS) publishes scientific contributions in the surfactants and detergents area. This includes the basic and applied science of petrochemical and oleochemical surfactants, the development and performance of surfactants in all applications, as well as the development and manufacture of detergent ingredients and their formulation into finished products.
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