A Combination of Catechin and Metformin: Its Preclinical Efficacy and Safety in STZ-Induced Diabetic Rats

IF 1.2 4区医学 Q4 CHEMISTRY, MEDICINAL

Letters in Drug Design & Discovery Pub Date : 2024-07-11 DOI:10.2174/0115701808304505240628103332

Jyoshna Rani Dash, Gurudutta Pattnaik, Ashirbad Nanda, Goutam Ghosh, Goutam Rath, Biswakanth Kar

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Abstract

Objective: This study aimed to assess the combinational antidiabetic effect of catechin and metformin in streptozotocin (STZ)-induced diabetic rats. Methods: Wistar rats were chosen and divided into five groups (n=6). STZ at the dose of 55 mg/kg was used intraperitoneally for the induction of diabetes. The combination of catechin (CTN) and metformin (MET) was administered to diabetic rats. The changes in fasting blood sugar, body weight, Hb, HbA1c, creatinine, lipid profiles (TC, HDL, LDL, and TG), biochemical parameters (SGOT, SGPT, and ALP), and endogenous antioxidant parameters (SOD, GSH, and catalase) were assessed. Histopathological study of the β-cells in islets of the pancreas, kidney tubules, and liver cells was conducted in all groups. Results: The result showed a significant reduction (p < 0.001) in blood sugar in the CTN and METtreated group compared to the control group. The combination of CTN (50 mg/kg) and MET (22.5 mg/kg) significantly restored the creatinine levels and urine volumes, SGOT, SGPT, and ALP, compared to a single administration dose. The abnormal lipid profile levels (TC, LDL, TG, and HDL) and antioxidant enzymes (SOD, GSH, catalase) in diabetic control rats were restored to average levels in a significant manner. Histopathological results revealed significant alterations, including hypertrophy of islets and mild degeneration, renal necrosis, and inflammation of hepatocytes. Conclusion: The findings indicate that a combination of therapy (CTN+MET) improved the protective effect of the pancreas, kidney, and liver, suggesting that the combination shows a potential antidiabetic effect.

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儿茶素和二甲双胍的复方制剂：在 STZ 诱导的糖尿病大鼠中的临床前疗效和安全性

研究目的本研究旨在评估儿茶素和二甲双胍对链脲佐菌素（STZ）诱导的糖尿病大鼠的联合抗糖尿病作用。研究方法选取 Wistar 大鼠，将其分为 5 组（n=6）。腹腔注射 55 mg/kg 剂量的 STZ 诱导糖尿病。给糖尿病大鼠服用儿茶素（CTN）和二甲双胍（MET）复方制剂。评估了空腹血糖、体重、血红蛋白、HbA1c、肌酐、血脂（TC、HDL、LDL 和 TG）、生化指标（SGOT、SGPT 和 ALP）以及内源性抗氧化指标（SOD、GSH 和过氧化氢酶）的变化。对所有组的胰岛β细胞、肾小管和肝细胞进行了组织病理学研究。结果显示结果显示，与对照组相比，CTN 和 MET 治疗组的血糖明显降低（p < 0.001）。与单次给药相比，CTN（50 毫克/千克）和 MET（22.5 毫克/千克）联合给药能显著恢复肌酐水平、尿量、SGOT、SGPT 和 ALP。糖尿病对照组大鼠的血脂异常水平（总胆固醇、低密度脂蛋白、总胆固醇和高密度脂蛋白）和抗氧化酶（SOD、谷胱甘肽、过氧化氢酶）均明显恢复到平均水平。组织病理学结果显示，大鼠的组织发生了明显变化，包括胰岛肥大和轻度变性、肾脏坏死以及肝细胞炎症。结论研究结果表明，联合疗法（CTN+MET）改善了对胰腺、肾脏和肝脏的保护作用，表明联合疗法具有潜在的抗糖尿病效果。

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来源期刊

Letters in Drug Design & Discovery 医学-医药化学

CiteScore

1.80

自引率

10.00%

发文量

245

审稿时长

3 months

期刊介绍： Aims & Scope Letters in Drug Design & Discovery publishes letters, mini-reviews, highlights and guest edited thematic issues in all areas of rational drug design and discovery including medicinal chemistry, in-silico drug design, combinatorial chemistry, high-throughput screening, drug targets, and structure-activity relationships. The emphasis is on publishing quality papers very rapidly by taking full advantage of latest Internet technology for both submission and review of manuscripts. The online journal is an essential reading to all pharmaceutical scientists involved in research in drug design and discovery.