Control of Staphylococcus epidermidis biofilm by surfactins of an endophytic bacterium Bacillus sp. 15 F

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2024-07-03 DOI:10.1016/j.enzmictec.2024.110477

Marwa Jardak , Raphaël Lami , Oumaima Saadaoui , Hajer Jlidi , Didier Stien , Sami Aifa , Sami Mnif

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引用次数: 0

Abstract

The present paper deals with the preparation and annotation of a surfactin(s) derived from a culture of the endophytic bacterium Bacillus 15 F. The LC-MS analysis of the acetonitrile fraction confirmed the presence of surfactins Leu/Ile7 C15, Leu/Ile7 C14 and Leu/Ile7 C13 with [M+H]⁺ at m/z 1036.6895, 1022.6741 and 1008.6581, respectively. Various concentrations of the surfactin(s) (hereafter referred to as surfactin-15 F) were used to reduce the adhesion of Staphylococcus epidermidis S61, which served as a model for studying antibiofilm activity on polystyrene surfaces. Incubation of Staphylococcus epidermidis S61 with 62.5 µg/ml of surfactin-15 F resulted in almost complete inhibition of biofilm formation (90.3 ± 3.33 %), and a significant reduction of cell viability (resazurin-based fluorescence was more than 200 times lower). The antiadhesive effect of surfactin-15 F was confirmed by scanning electron microscopy. Surfactin-15 F demonstrated an eradication effect against preformed biofilm, causing severe disruption of Staphylococcus epidermidis S61 biofilm structure and reducing viability. The results suggest that surfactins produced by endophytic bacteria could be an alternative to synthetic products. Surfactin-15 F, used in wound dressings, demonstrated an efficient treatment of the preformed Staphylococcus epidermidis S61 biofilm, and thus having a great potential in medical applications.

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内生细菌芽孢杆菌的表面活性剂对表皮葡萄球菌生物膜的控制 15 F

本文论述了从内生芽孢杆菌 15 F 培养物中提取的表面活性剂的制备和注释。乙腈馏分的 LC-MS 分析证实了表面活性剂 Leu/Ile7 C15、Leu/Ile7 C14 和 Leu/Ile7 C13 的存在，[M+H]+ 分别为 m/z 1036.6895、1022.6741 和 1008.6581。不同浓度的表面活性剂（以下简称为表面活性剂-15 F）可用于减少表皮葡萄球菌 S61 的粘附，S61 是研究聚苯乙烯表面抗生物膜活性的模型。用 62.5 µg/ml 的表面活性剂-15 F 培养表皮葡萄球菌 S61，几乎完全抑制了生物膜的形成（90.3 ± 3.33 %），并显著降低了细胞的活力（基于利马唑啉的荧光降低了 200 多倍）。扫描电子显微镜证实了表面活性剂-15 F 的抗粘附效果。表面活性剂-15 F 对预先形成的生物膜具有根除作用，可严重破坏表皮葡萄球菌 S61 的生物膜结构并降低其存活率。结果表明，内生细菌产生的表面活性剂可以替代合成产品。用于伤口敷料的 Surfactin-15 F 能有效处理已形成的表皮葡萄球菌 S61 生物膜，因此在医疗应用方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.