Green synthesis of silver nanoparticles using food supplement from Avena sativa L., and their antioxidant, antiglycation, and anti-aging activities: In vitro and in silico studies

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Bianca Silva Bras , Isabelly do Nascimento Pereira , Laura Camargo Zibordi , Pedro Augusto Pereira Rosatto , Hugo Henrique Santos , Filipe Oliveira Granero , Célia Cristina Malaguti Figueiredo , Mary Leiva de Faria , Valdecir Farias Ximenes , Rodolfo Osin de Moraes , Patrícia Soares Santiago , Nilson Nicolau-Junior , Luciana Pereira Silva , Regildo Márcio Gonçalves Silva
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引用次数: 0

Abstract

Food supplement of Avena sativa L. dry extract (AsDE) is produced with green leaves of wild oats and it is commonly used in patients with cognitive disorders and muscle deficits. These symptoms and pathologies can be promoted and/or aggravated by oxidative stress and protein glycation. In order to prevent or treat these disorders, new formulations that could enhance and benefit AsDE have been developed and the formation of metallic nanoparticles by green synthesis has shown to be an alternative. Thus, this study aimed to prepare silver nanoparticles (AgNPs) by green synthesis using AsDE and characterize these AgNPs. Besides, it aimed to evaluate in vitro antioxidant and antiglycation activities of AsDE and AgNPS, and analyze in vitro and in silico inhibitory action on aging enzymes (collagenase, elastase, and tyrosinase). UV–visible spectroscopy, Zeta potential and scanning transmission electron microscopy were used to characterize AgNPs. Antioxidant activity was determined by DPPH free radical scavenging, ferric ion reducing power (FRAP), lipid peroxidation inhibition, ABTS radical scavenging, and oxygen radical absorption capacity (ORAC). Molecular docking analyses were performed to evaluate interactions between the major compounds of AsDE and aging enzymes or DNA. Antioxidant tests demonstrated antioxidant activity of 19.67 % for AsDE and 42.98 % for AgNPs in DPPH test; and 115.67 µM Trolox Equivalent (TE)/g for AsDE and 554.33 µM TE/g for AgNPs in FRAP test. In addition, AsDE and AgNPs inhibited 25.40 % and 11.33 % of lipid peroxidation, respectively, and presented 255.22 µM TE/g for AsDE and 317.44 µM TE/g for AgNPs in ABTS test. AsDE and AgNPs exhibited antioxidant potential observed in ORAC assay. In addition, AsDE presented inhibitory action on collagenase, elastase and tyrosinase activities (78.23 %, 68.14 % and 62.27 % inhibition, respectively). Antiglycation tests demonstrated that bovine serum albumin exposed to ribose and treated with AsDE at 0.5 mg/mL exhibited the highest percentage of free amino groups (45.21 %), while samples treated with AgNPs showed 35.41 % free amino groups. On the other hand, AgNPs exhibited the highest percentage of inhibition of advanced glycation end-products formation (85.75 %), which did not differ from aminoguanidine, a known antiglycation agent. Besides, relative electrophoretic mobility assay demonstrated that samples treated with AsDE at 0.1 mg/mL or AgNPs showed antiglycation activity comparable to aminoguanidine. The in silico assays showed interactions between the major compounds of AsDE and aging enzymes or DNA. Therefore, results represent an important indicator for the development and discovery of new nanostructured pharmaceutical and cosmetic formulations using plants and their bioactive compounds.

Abstract Image

利用从莜麦(Avena sativa L.)中提取的食品添加剂绿色合成银纳米粒子及其抗氧化、抗糖化和抗衰老活性:体外和硅学研究
燕麦干提取物(AsDE)是用野生燕麦的绿叶制成的食品补充剂,常用于患有认知障碍和肌肉缺陷的患者。氧化应激和蛋白质糖化可促进和/或加重这些症状和病理变化。为了预防或治疗这些疾病,人们开发出了可增强和有益于 AsDE 的新配方,而通过绿色合成形成金属纳米粒子已被证明是一种替代方法。因此,本研究旨在利用 AsDE 通过绿色合成制备银纳米粒子(AgNPs),并对这些 AgNPs 进行表征。此外,本研究还旨在评估 AsDE 和 AgNPS 的体外抗氧化和抗糖化活性,并分析其对衰老酶(胶原酶、弹性蛋白酶和酪氨酸酶)的体外和体内抑制作用。利用紫外可见光谱、Zeta 电位和扫描透射电子显微镜对 AgNPs 进行表征。抗氧化活性通过 DPPH 自由基清除、铁离子还原力(FRAP)、脂质过氧化抑制、ABTS 自由基清除和氧自由基吸收能力(ORAC)进行测定。进行了分子对接分析,以评估 AsDE 的主要化合物与衰老酶或 DNA 之间的相互作用。抗氧化测试表明,在DPPH测试中,AsDE的抗氧化活性为19.67%,AgNPs为42.98%;在FRAP测试中,AsDE的抗氧化活性为115.67 µM Trolox Equivalent (TE)/g,AgNPs为554.33 µM TE/g。此外,AsDE 和 AgNPs 还分别抑制了 25.40 % 和 11.33 % 的脂质过氧化反应,在 ABTS 测试中,AsDE 和 AgNPs 分别呈现出 255.22 µM TE/g 和 317.44 µM TE/g。在 ORAC 试验中观察到 AsDE 和 AgNPs 具有抗氧化潜力。此外,AsDE 还对胶原酶、弹性蛋白酶和酪氨酸酶活性具有抑制作用(抑制率分别为 78.23%、68.14% 和 62.27%)。抗糖化测试表明,暴露于核糖并用 0.5 毫克/毫升 AsDE 处理的牛血清白蛋白显示出最高百分比的游离氨基(45.21%),而用 AgNPs 处理的样品显示出 35.41% 的游离氨基。另一方面,AgNPs 对高级糖化终产物形成的抑制率最高(85.75%),与已知的抗糖化剂氨基胍没有差别。此外,相对电泳迁移率测定表明,用 0.1 毫克/毫升的 AsDE 或 AgNPs 处理的样品具有与氨基胍相当的抗糖化活性。硅学分析表明,AsDE 的主要化合物与衰老酶或 DNA 之间存在相互作用。因此,研究结果是利用植物及其生物活性化合物开发和发现新型纳米结构药物和化妆品配方的重要指标。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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