{"title":"Additional use of α-IgM antibodies potentiates CpG ODN2006-induced B cell activation by targeting mainly naïve and marginal zone-like B cells","authors":"Leonie Fleige , Simon Fillatreau , Maren Claus , Silvia Capellino","doi":"10.1016/j.cellimm.2024.104846","DOIUrl":null,"url":null,"abstract":"<div><p>CpG ODN2006 is widely used as a potent B cell stimulant <em>in vitro</em> and <em>in vivo</em>. However, it shows a deficit in targeting naïve B cells <em>in vitro</em>. In this study, we investigated whether α-IgM can support ODN2006-induced effects on B cells to obtain enhanced activation with focus on different B cell subsets.</p><p>Our results delineated robust B cell activation, shown by increased activation marker expression and cytokine secretion by each agent alone, and further augmented when used in combination. Interestingly, α-IgM targeted mainly naïve and marginal zone-like B cells, thus complementing the pronounced effects of ODN2006 on memory B cells and achieving optimal activation for all B cell subsets.</p><p>Taken together, combining ODN2006 and α-IgM is beneficial for <em>in vitro</em> activation including all B cell subsets. Furthermore, our results suggest that α-IgM could enhance efficacy of ODN2006 <em>in vivo</em> with further need of investigation.</p></div>","PeriodicalId":9795,"journal":{"name":"Cellular immunology","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008874924000492","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
CpG ODN2006 is widely used as a potent B cell stimulant in vitro and in vivo. However, it shows a deficit in targeting naïve B cells in vitro. In this study, we investigated whether α-IgM can support ODN2006-induced effects on B cells to obtain enhanced activation with focus on different B cell subsets.
Our results delineated robust B cell activation, shown by increased activation marker expression and cytokine secretion by each agent alone, and further augmented when used in combination. Interestingly, α-IgM targeted mainly naïve and marginal zone-like B cells, thus complementing the pronounced effects of ODN2006 on memory B cells and achieving optimal activation for all B cell subsets.
Taken together, combining ODN2006 and α-IgM is beneficial for in vitro activation including all B cell subsets. Furthermore, our results suggest that α-IgM could enhance efficacy of ODN2006 in vivo with further need of investigation.
额外使用 α-IgM 抗体可通过主要靶向幼稚和边缘区样 B 细胞，增强 CpG ODN2006 诱导的 B 细胞活化作用
CpG ODN2006 被广泛用作体外和体内的强效 B 细胞刺激剂。然而，它在体外靶向幼稚 B 细胞方面存在缺陷。在这项研究中，我们研究了 α-IgM 是否能支持 ODN2006 诱导的对 B 细胞的作用，从而增强对不同 B 细胞亚群的激活。有趣的是，α-IgM 主要针对幼稚和边缘区样 B 细胞，从而补充了 ODN2006 对记忆 B 细胞的明显作用，并实现了所有 B 细胞亚群的最佳活化。此外，我们的研究结果表明，α-IgM 可以提高 ODN2006 在体内的疗效，但还需要进一步研究。
期刊介绍：
Cellular Immunology publishes original investigations concerned with the immunological activities of cells in experimental or clinical situations. The scope of the journal encompasses the broad area of in vitro and in vivo studies of cellular immune responses. Purely clinical descriptive studies are not considered.
Research Areas include:
• Antigen receptor sites
• Autoimmunity
• Delayed-type hypersensitivity or cellular immunity
• Immunologic deficiency states and their reconstitution
• Immunologic surveillance and tumor immunity
• Immunomodulation
• Immunotherapy
• Lymphokines and cytokines
• Nonantibody immunity
• Parasite immunology
• Resistance to intracellular microbial and viral infection
• Thymus and lymphocyte immunobiology
• Transplantation immunology
• Tumor immunity.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
