Specific Mode Electroacupuncture Stimulation Mediates the Delivery of NGF Across the Hippocampus Blood-Brain Barrier Through p65-VEGFA-TJs to Improve the Cognitive Function of MCAO/R Convalescent Rats.

IF 4.6 2区医学 Q1 NEUROSCIENCES

Molecular Neurobiology Pub Date : 2025-02-01 Epub Date: 2024-07-12 DOI:10.1007/s12035-024-04337-8

Mengyuan Dai, Kecheng Qian, Qinyu Ye, Jinding Yang, Lin Gan, Zhaoxing Jia, Zixing Pan, Qian Cai, Tianxiang Jiang, Congcong Ma, Xianming Lin

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引用次数: 0

Abstract

Cognitive impairment frequently presents as a prevalent consequence following stroke, imposing significant burdens on patients, families, and society. The objective of this study was to assess the effectiveness and underlying mechanism of nerve growth factor (NGF) in treating post-stroke cognitive dysfunction in rats with cerebral ischemia-reperfusion injury (MCAO/R) through delivery into the brain using specific mode electroacupuncture stimulation (SMES). From the 28th day after modeling, the rats were treated with NGF mediated by SMES, and the cognitive function of the rats was observed after treatment. Learning and memory ability were evaluated using behavioral tests. The impact of SMES on blood-brain barrier (BBB) permeability, the underlying mechanism of cognitive enhancement in rats with MCAO/R, including transmission electron microscopy, enzyme-linked immunosorbent assay, immunohistochemistry, immunofluorescence, and TUNEL staining. We reported that SMES demonstrates a safe and efficient ability to open the BBB during the cerebral ischemia repair phase, facilitating the delivery of NGF to the brain by the p65-VEGFA-TJs pathway.

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特定模式电针刺激通过p65-VEGFA-TJs介导NGF穿过海马血脑屏障改善MCAO/R康复大鼠的认知功能

认知功能障碍是脑卒中后的常见后果，给患者、家庭和社会带来沉重负担。本研究的目的是评估神经生长因子（NGF）通过特定模式电针刺激（SMES）送入脑部治疗脑缺血再灌注损伤（MCAO/R）大鼠卒中后认知功能障碍的有效性及其内在机制。从建模后第 28 天开始，大鼠接受由特定模式电针刺激介导的 NGF 治疗，并观察治疗后大鼠的认知功能。通过行为测试评估了大鼠的学习和记忆能力。SMES对血脑屏障（BBB）通透性的影响、MCAO/R大鼠认知能力增强的内在机制，包括透射电子显微镜、酶联免疫吸附试验、免疫组织化学、免疫荧光和TUNEL染色。我们报告说，在脑缺血修复阶段，SMES能安全有效地打开BBB，促进NGF通过p65-VEGFA-TJs途径输送到大脑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Neurobiology 医学-神经科学

CiteScore

9.00

自引率

2.00%

发文量

480

审稿时长

1 months

期刊介绍： Molecular Neurobiology is an exciting journal for neuroscientists needing to stay in close touch with progress at the forefront of molecular brain research today. It is an especially important periodical for graduate students and "postdocs," specifically designed to synthesize and critically assess research trends for all neuroscientists hoping to stay active at the cutting edge of this dramatically developing area. This journal has proven to be crucial in departmental libraries, serving as essential reading for every committed neuroscientist who is striving to keep abreast of all rapid developments in a forefront field. Most recent significant advances in experimental and clinical neuroscience have been occurring at the molecular level. Until now, there has been no journal devoted to looking closely at this fragmented literature in a critical, coherent fashion. Each submission is thoroughly analyzed by scientists and clinicians internationally renowned for their special competence in the areas treated.