Distribution of active ingredients and quality control of Forsythia suspensa with AP-MALDI mass spectrometry imaging

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Yongli Liu, Cheng Wang, Zhenhe Chen, Hao Yuan, Rong Lei, Xiaodong Li, Shuangcheng Ma, Changxiao Liu
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引用次数: 0

Abstract

The fruits of Forsythia suspensa (F. suspensa) have been used as a traditional Chinese medicine for 2000 years. Currently, the quality control of F. suspensa strictly follows the instructions of Chinese Pharmacopeia, which mainly controls the content of forsythoside A, phillyrin, and volatile oil. In this study, air pressure MALDI mass spectrometry imaging (AP-MALDI MSI) was used to evaluate the quality of F. suspensa fruits and the distribution of dozens of active ingredients. The variation of active ingredients was measured for more than 30 batches of samples, regarding harvest time, cultivated environment, shelf-life, and habitat. Fifty-three active ingredients could be detected in F. suspensa fruits with AP-MALDI MSI. Seven active ingredients were upregulated, four ingredients downregulated, and 15 ingredients did not change in ripe fruits. A sharp variation of active ingredients in late September was observed for the Caochuan fruits harvested in 2019, which is closely related to the appearance of the ginger color of the pericarp under the microscope observation. The microscope observation is a reliable way to classify ripe and green fruits instead of outlook. Just considering forsythoside A and phillyrin, it is found that wild fruits are better than cultivated fruits, but cultivated fruits have high contents of other ingredients. The shelf-life of F. suspensa fruits is proposed to be 3 years, considering the 26 ingredients investigated. It was found that Luoning wild fruits are better than those from Caochuan with a new evaluation method. Mass spectrometry imaging is an easy, objective, and effective method to evaluate the quality of F. suspensa fruits.

利用 AP-MALDI 质谱成像技术对悬钩子连翘的有效成分进行分布和质量控制。
连翘果实作为传统中药已有 2000 年的历史。目前,连翘的质量控制严格按照《中国药典》的要求进行,主要控制连翘苷 A、菲林和挥发油的含量。本研究采用气压 MALDI 质谱成像技术(AP-MALDI MSI)对悬钩子果实的质量及数十种有效成分的分布进行了评价。根据收获时间、栽培环境、货架期和栖息地,对 30 多批样品的有效成分变化进行了测定。通过 AP-MALDI MSI,可以在悬铃木果实中检测到 53 种活性成分。在成熟的果实中,7 种活性成分上调,4 种成分下调,15 种成分没有变化。2019 年采收的草川果实在 9 月下旬出现了活性成分的急剧变化,这与显微镜观察下果皮姜黄色的出现密切相关。显微镜观察是划分成熟果和青果的可靠方法,而不是外观。仅从连翘苷 A 和菲力蛋白的角度来看,野生果实比栽培果实好,但栽培果实的其他成分含量高。考虑到所调查的 26 种成分,建议悬钩子果实的货架期为 3 年。通过一种新的评价方法发现,洛宁野果的品质优于草川野果。质谱成像是评价悬钩子果质量的一种简便、客观、有效的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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