Sensitive Detection of Umami Substances in Meat with Synthesized Electrochemical Sensor Enhanced by MXene Surface-Doped Peroxidase-Mimic Nanoenzymes (Fe3O4)
{"title":"Sensitive Detection of Umami Substances in Meat with Synthesized Electrochemical Sensor Enhanced by MXene Surface-Doped Peroxidase-Mimic Nanoenzymes (Fe3O4)","authors":"Yingkun Shi, Zijun Niu, Sichun Chen, Songlei Wang, Lingfan Yang, Yihua Wang","doi":"10.1007/s11947-024-03507-9","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Electrochemical analysis is a powerful analytical technique with extensive applications in the food industry, offers high advantages due to its high sensitivity, low operating cost, and high-speed analysis. In this study, a novel nanoenzymes high-catalytic platform based on a conductive polymer (MXene) was established for the detection of Guanosine 5′-monophosphate (GMP). The MXene/Fe<sub>3</sub>O<sub>4</sub>/β-CD nanocomposites were synthesized using a self-assembly method, exhibiting significant catalytic activity and a large specific surface area. The surface morphology, functional groups and chemical bonds of MXene/Fe<sub>3</sub>O<sub>4</sub>/β-CD were analyzed via scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Electrochemical experiments indicated a significant amplification of GMP electrical signals on the nanoenzymatic platform. The oxidation of GMP to 8-oxo-GMP involved the transfer of two electrons (−2e<sup>−</sup>) and two protons (-2H<sup>+</sup>) at the MXene/Fe<sub>3</sub>O<sub>4</sub>/β-CD modified glassy carbon electrode. Under optimized conditions, this electrochemical nanoenzymatic platform achieved a low detection limit of 0.0126 μM for GMP, with linear ranges from 0.05 μM to 600 μM. The sensor demonstrated excellent selectivity and stability and was successfully applied for GMP determination in chicken, fish, pork and beef samples. This study provided a new pathway for the development of sensors for GMP detection.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":562,"journal":{"name":"Food and Bioprocess Technology","volume":"175 1","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioprocess Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11947-024-03507-9","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Electrochemical analysis is a powerful analytical technique with extensive applications in the food industry, offers high advantages due to its high sensitivity, low operating cost, and high-speed analysis. In this study, a novel nanoenzymes high-catalytic platform based on a conductive polymer (MXene) was established for the detection of Guanosine 5′-monophosphate (GMP). The MXene/Fe3O4/β-CD nanocomposites were synthesized using a self-assembly method, exhibiting significant catalytic activity and a large specific surface area. The surface morphology, functional groups and chemical bonds of MXene/Fe3O4/β-CD were analyzed via scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Electrochemical experiments indicated a significant amplification of GMP electrical signals on the nanoenzymatic platform. The oxidation of GMP to 8-oxo-GMP involved the transfer of two electrons (−2e−) and two protons (-2H+) at the MXene/Fe3O4/β-CD modified glassy carbon electrode. Under optimized conditions, this electrochemical nanoenzymatic platform achieved a low detection limit of 0.0126 μM for GMP, with linear ranges from 0.05 μM to 600 μM. The sensor demonstrated excellent selectivity and stability and was successfully applied for GMP determination in chicken, fish, pork and beef samples. This study provided a new pathway for the development of sensors for GMP detection.
期刊介绍：
Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community.
The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
