Electrochemical Immunoassay Using Diffusion Layer Dispersion Effect of Electrodes

IF 5.5 3区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Eunyoung Kim, Jun-Hee Park, Bong-Ki Ryu, Ga-Yeon Lee
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Abstract

Diffusion layer dispersed electrodes were presented for application in electrochemical immunoassays. The diffusion layer refers to the region on the electrode surface where concentration gradients occur during the redox reactions of the target analytes. To control the diffusion layer, electrodes of the same area and size were fabricated, and the shape of the diffusion layer was controlled by adjusting each electrode distance. To estimate the properties of the electrodes, cyclic voltammetry (CV) analysis was performed using ferricyanide ([Fe(CN)6–3]) and 3,3′5,5′-tetramethylbenzidine (TMB) as a model redox couple. Changes in the diffusion layer shape according to electrode distance were compared through simulation. The redox currents for the target analytes increased as electrode distance increased. These results indicate that even if the electrode shape and area are the same, dispersing the diffusion layer by adjusting the distance between the electrodes can affect the microelectrode characteristics. Finally, the diffusion layer dispersed electrode was applied for the medical diagnosis of the human hepatitis B virus (hHBV) antigen, and the human hepatitis C virus (hHCV) antibody was compared with conventional assay methods, such as TMB-based chromogenic detection.

Abstract Image

利用电极的扩散层分散效应进行电化学免疫测定
扩散层分散电极被应用于电化学免疫分析。扩散层是指目标分析物发生氧化还原反应时,电极表面出现浓度梯度的区域。为了控制扩散层,我们制作了相同面积和尺寸的电极,并通过调整每个电极的距离来控制扩散层的形状。为了估算电极的特性,使用三氯化铁([Fe(CN)6-3])和 3,3′5,5′-四甲基联苯胺(TMB)作为模型氧化还原偶,进行了循环伏安法(CV)分析。通过模拟比较了扩散层形状随电极距离的变化。目标分析物的氧化还原电流随着电极距离的增加而增加。这些结果表明,即使电极的形状和面积相同,通过调整电极之间的距离来分散扩散层也会影响微电极的特性。最后,将扩散层分散电极应用于人类乙型肝炎病毒(hHBV)抗原和人类丙型肝炎病毒(hHCV)抗体的医学诊断,并与基于 TMB 的色原检测等传统检测方法进行了比较。
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来源期刊
BioChip Journal
BioChip Journal 生物-生化研究方法
CiteScore
7.70
自引率
16.30%
发文量
47
审稿时长
6-12 weeks
期刊介绍: BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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