Exogenous Glutathione Enhances Salt Tolerance in Kenaf by Mediating Modulation of Oxidative Stress Response and DNA Methylation

IF 3.9 3区生物学 Q1 PLANT SCIENCES

Journal of Plant Growth Regulation Pub Date : 2024-07-09 DOI:10.1007/s00344-024-11402-y

Shan Cao, Guowang Liang, Lixia Zhang, Jiao Pan, Ru Li, Peng Chen

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Abstract

Kenaf (Hibiscus cannabinus L.) is an important fiber crop, which can be applied for the restoration of saline-alkali land. The objective of our study was to investigate the impacts of exogenous glutathione (GSH) on physiological and biochemical properties, ion balance, and DNA methylation of kenaf under salt stress. We used Hoagland nutrient solution containing 200 mM NaCl to simulate salt stress, and found the growth of kenaf seedlings was substantially hindered. 100 μM GSH pretreatment effectively increased the plant height, stem diameter, main root length, and fresh weight under salt stress, as well as reduced the uptake of Na⁺ and Cl⁻ and promoted the uptake of K⁺. Besides, exogenous GSH pretreatment protected kenaf plants from salt-induced adversities by reducing the ROS-induced oxidative damage, enhancing the contents of chlorophyll, proline, and soluble sugar. Salinity reduced the total DNA methylation level in kenaf genome, triggering higher mRNA expressions of HcGLP3, HcDOF1.4, HcULP3, HcVHA, HcPP2C39, and HcSRF6. However, GSH addition enhanced the total DNA methylation level. We further utilized virus-induced genes silencing technique to confirm that HcGLP3 played a positive role in the response of kenaf to salinity. Taken together, exogenous GSH could enhance salt tolerance in kenaf by mediating modulation of oxidative stress response and DNA methylation.

Graphical Abstract

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外源谷胱甘肽通过调节氧化应激反应和 DNA 甲基化增强剑麻的耐盐性

木槿（Hibiscus cannabinus L.）是一种重要的纤维作物，可用于盐碱地的恢复。我们的研究旨在探讨外源谷胱甘肽（GSH）对盐胁迫下剑麻的生理生化特性、离子平衡和 DNA 甲基化的影响。我们使用含有 200 mM NaCl 的 Hoagland 营养液模拟盐胁迫，发现剑麻幼苗的生长受到严重阻碍。100 μM GSH预处理可有效增加盐胁迫下的株高、茎径、主根长度和鲜重，并减少对Na+和Cl-的吸收，促进对K+的吸收。此外，外源 GSH 预处理可减少 ROS 诱导的氧化损伤，提高叶绿素、脯氨酸和可溶性糖的含量，从而保护红豆杉免受盐胁迫。盐分降低了红景天基因组的 DNA 甲基化总水平，导致 HcGLP3、HcDOF1.4、HcULP3、HcVHA、HcPP2C39 和 HcSRF6 的 mRNA 表达量升高。然而，GSH的添加提高了总DNA甲基化水平。我们进一步利用病毒诱导基因沉默技术证实，HcGLP3 在剑麻对盐度的反应中发挥了积极作用。综上所述，外源 GSH 可通过调节氧化应激反应和 DNA 甲基化提高剑麻的耐盐性。

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来源期刊

Journal of Plant Growth Regulation 生物-植物科学

CiteScore

8.40

自引率

6.20%

发文量

312

审稿时长

1.8 months

期刊介绍： The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.