Host plants expressing pxy-miR-34-5p demonstrate high efficacy against multiple lepidopterous pests

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-07-04 DOI:10.1127/entomologia/2024/2416

Linhong Li, Tianxin Guo, Bin Zhu, Pei Liang

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引用次数: 0

Abstract

Lepidopteran pests are destructive pests and have evolved significant resistance to many insecticides. Alternative green control strategies are urgently needed. Our previous research had revealed that overexpression or knockdown of a Plutella xylostella microRNA, pxy-miR-34-5p, could lead to high mortality and severe developmental defects in multiple lepidopteran pests, thus has great potential in lepidopteran pests control. Here, pxy-miR-34-5p was successfully expressed in cruciferous plants, Arabidopsis thaliana and Brassica campestris. Feeding on leaves of transgenic miR-34-5p rape significantly reduced the expression of ecdysone receptor gene PxEcR of P. xylostella by 56.8–70.2%. Bioassays results demonstrated that compared to that of the larvae fed on wild type Arabidopsis, the accumulated mortalities of P. xylostella fed on transgenic miR-34-5p Arabidopsis were significantly increased by 34.9%–52.0%. After feeding on transgenic miR-34-5p rape leaves for four to six days, the highest accumulated corrected mortalities of P. xylostella, Spodoptera frugiperda and Spodoptera exigua larvae reached 100.0%, 94.1%, and 100.0%, respectively. These results indicated that insect microRNAs can be used to control multiple target pests by constructing transgenic crops, which lays an important foundation for the further development of miRNA-based green pest control strategies.

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表达 pxy-miR-34-5p 的寄主植物对多种鳞翅目害虫具有很高的防治效果

鳞翅目害虫是毁灭性害虫，对许多杀虫剂都产生了严重的抗药性。我们迫切需要替代性的绿色控制策略。我们之前的研究发现，过表达或敲除一种名为pxy-miR-34-5p的鳞翅目害虫微RNA（Plutella xylostella microRNA）可导致多种鳞翅目害虫的高死亡率和严重发育缺陷，因此在鳞翅目害虫防治中具有巨大潜力。在此，pxy-miR-34-5p成功地在十字花科植物拟南芥和甘蓝中表达。以转基因 miR-34-5p 油菜叶片为食，可显著降低木虱蜕皮激素受体基因 PxEcR 的表达量 56.8-70.2%。生物测定结果表明，与喂食野生型拟南芥的幼虫相比，喂食转基因 miR-34-5p 拟南芥的 P. xylostella 的累积死亡率明显增加了 34.9%-52.0% 。在取食转基因 miR-34-5p 油菜叶片 4-6 天后，木虱、鞘翅目和鞘翅目幼虫的最高累积校正死亡率分别达到 100.0%、94.1% 和 100.0%。这些结果表明，通过构建转基因作物，昆虫微RNA可用于控制多种靶标害虫，这为进一步发展基于miRNA的害虫绿色防控策略奠定了重要基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.