The protective effect of pregabalin and xanthenone on testicular ischemia/reperfusion injury in rats

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY

Fundamental & Clinical Pharmacology Pub Date : 2024-07-08 DOI:10.1111/fcp.13027

Maha M. Abdel-Fattah, Ahmed Mamdouh Ahmed, Rabeh Khairy Saleh, Basim Anwar Shehata Messiha, Remon Roshdy Rofaeil

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引用次数: 0

Abstract

Background

Torsion of the spermatic cord is a hazardous and common urologic issue. The current work evaluates the possible protective effect of pregabalin (PGB) and xanthenone (XAN) in testicular ischemia/reperfusion injury induced by testicular torsion/detorsion in rats.

Materials and methods

Seven groups of adult male Wistar albino rats were allocated randomly into seven groups, namely, sham control, torsion/detorsion (T/D), PGB 50 mg/kg, PGB 100 mg/kg, XAN 1 mg/kg, XAN 2 mg/kg, and PGB 50 mg/kg plus XAN 1 mg/kg groups. Serum cholesterol and testosterone levels were determined. Also, the levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α), nuclear factor kappa B (NF-қB), angiotensin (Ang) II, Ang-(1–7), and angiotensin-converting enzyme2 (ACE2) were assessed in testicular tissue. Immunohistochemical analysis of heme oxygenase-1 (HO-1) and caspase-3 was performed. Finally, the histopathological examination of the testicular tissues was performed.

Results

The PGB 50 mg/kg, PGB 100 mg/kg, XAN 1 mg/kg, XAN 2 mg/kg, and PGB 50 mg/kg plus XAN 1 mg/kg groups showed a significant decrease in serum cholesterol, MDA, NO, TNF-α, NF-қB, and Ang-II levels coupled with a significant increase in both testosterone and ACE2 expression. Furthermore, all test groups showed a significant improvement in the histopathological picture with a reduction in caspase-3 and an increase in HO-1 immunoexpression in testicular tissue.

Conclusion

PGB and XAN may have promising effects on preventing testicular T/D injury through antioxidant, anti-inflammatory, and antiapoptotic actions.

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普瑞巴林和香丹酮对大鼠睾丸缺血再灌注损伤的保护作用

背景：精索扭转是泌尿科常见的危险问题。本研究评估了普瑞巴林（PGB）和安体舒通（XAN）对大鼠睾丸扭转/离体诱导的睾丸缺血/再灌注损伤可能具有的保护作用：将7组成年雄性Wistar白化大鼠随机分为7组，即假性对照组、扭转/离体（T/D）组、PGB 50 mg/kg组、PGB 100 mg/kg组、XAN 1 mg/kg组、XAN 2 mg/kg组和PGB 50 mg/kg加XAN 1 mg/kg组。测定了血清胆固醇和睾酮水平。此外，还评估了睾丸组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）、一氧化氮（NO）、超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）、核因子卡巴B（NF-қB）、血管紧张素（Ang）II、Ang-（1-7）和血管紧张素转换酶2（ACE2）的水平。对血红素加氧酶-1（HO-1）和Caspase-3进行了免疫组化分析。最后，对睾丸组织进行了组织病理学检查：结果：PGB 50 mg/kg组、PGB 100 mg/kg组、XAN 1 mg/kg组、XAN 2 mg/kg组和PGB 50 mg/kg加XAN 1 mg/kg组的血清胆固醇、MDA、NO、TNF-α、NF-қB和Ang-II水平显著下降，睾酮和ACE2的表达显著增加。此外，所有试验组的组织病理学状况都有明显改善，睾丸组织中的 Caspase-3 减少，HO-1 免疫表达增加：结论：PGB 和 XAN 可通过抗氧化、抗炎和抗细胞凋亡作用预防睾丸 T/D 损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fundamental & Clinical Pharmacology 医学-药学

CiteScore

5.30

自引率

6.90%

发文量

111

审稿时长

6-12 weeks

期刊介绍： Fundamental & Clinical Pharmacology publishes reports describing important and novel developments in fundamental as well as clinical research relevant to drug therapy. Original articles, short communications and reviews are published on all aspects of experimental and clinical pharmacology including: Antimicrobial, Antiviral Agents Autonomic Pharmacology Cardiovascular Pharmacology Cellular Pharmacology Clinical Trials Endocrinopharmacology Gene Therapy Inflammation, Immunopharmacology Lipids, Atherosclerosis Liver and G-I Tract Pharmacology Metabolism, Pharmacokinetics Neuropharmacology Neuropsychopharmacology Oncopharmacology Pediatric Pharmacology Development Pharmacoeconomics Pharmacoepidemiology Pharmacogenetics, Pharmacogenomics Pharmacovigilance Pulmonary Pharmacology Receptors, Signal Transduction Renal Pharmacology Thrombosis and Hemostasis Toxicopharmacology Clinical research, including clinical studies and clinical trials, may cover disciplines such as pharmacokinetics, pharmacodynamics, pharmacovigilance, pharmacoepidemiology, pharmacogenomics and pharmacoeconomics. Basic research articles from fields such as physiology and molecular biology which contribute to an understanding of drug therapy are also welcomed.