Fitness costs of Tn1546-type transposons harboring the vanA operon by plasmid type and structural diversity in Enterococcus faecium.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Dokyun Kim, Da Young Kang, Min Hyuk Choi, Jun Sung Hong, Hyun Soo Kim, Young Ree Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Jeong Hwan Shin, Soo Hyun Kim, Jong Hee Shin, Seok Hoon Jeong
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Abstract

Background: This study analyzed the genetic traits and fitness costs of vancomycin-resistant Enterococcus faecium (VREfm) blood isolates carrying Tn1546-type transposons harboring the vanA operon.

Methods: All E. faecium blood isolates were collected from eight general hospitals in South Korea during one-year study period. Antimicrobial susceptibility testing and vanA and vanB PCR were performed. Growth rates of E. faecium isolates were determined. The vanA-positive isolates were subjected to whole genome sequencing and conjugation experiments.

Results: Among 308 E. faecium isolates, 132 (42.9%) were positive for vanA. All Tn1546-type transposons harboring the vanA operon located on the plasmids, but on the chromosome in seven isolates. The plasmids harboring the vanA operon were grouped into four types; two types of circular, nonconjugative plasmids (Type A, n = 50; Type B, n = 46), and two types of putative linear, conjugative plasmids (Type C, n = 16; Type D, n = 5). Growth rates of vanA-positive E. faecium isolates were significantly lower than those of vanA-negative isolates (P < 0.001), and reduction in growth rate under vancomycin pressure was significantly larger in isolates harboring putative linear plasmids than in those harboring circular plasmids (P = 0.020).

Conclusions: The possession of vanA operon was costly to bacterial hosts in antimicrobial-free environment, which provide evidence for the importance of reducing vancomycin pressure for prevention of VREfm dissemination. Fitness burden to bacterial hosts was varied by type and size of the vanA operon-harboring plasmid.

粪肠球菌中携带 vanA 操作子的 Tn1546 型转座子因质粒类型和结构多样性而产生的健存成本。
背景:本研究分析了耐万古霉素粪肠球菌(VREfm)血液分离株的遗传特征和适应性成本,这些分离株携带有携带有 vanA 操作子的 Tn1546 型转座子:方法:在为期一年的研究期间,从韩国八家综合医院收集所有粪肠球菌血液分离物。方法:在为期一年的研究期间,从韩国 8 家综合医院收集了所有分离到的粪大肠杆菌,并进行了抗菌药敏感性测试以及 vanA 和 vanB PCR 检测。测定了分离出的粪肠球菌的生长率。对 vanA 阳性的分离株进行了全基因组测序和连接实验:结果:在 308 株粪肠球菌分离株中,有 132 株(42.9%)vanA 阳性。所有携带 vanA 操作子的 Tn1546 型转座子都位于质粒上,但有 7 个分离株的转座子位于染色体上。携带 vanA 操作子的质粒分为四类:两类环状非共轭质粒(A 型,n = 50;B 型,n = 46)和两类假定线性共轭质粒(C 型,n = 16;D 型,n = 5)。vanA阳性的粪肠球菌分离物的生长率明显低于vanA阴性的分离物(P 结论):在无抗菌素环境中,拥有 vanA 操作子的细菌宿主代价高昂,这证明了减少万古霉素压力对预防 VREfm 传播的重要性。vanA操作子载体质粒的类型和大小不同,对细菌宿主造成的健康负担也不同。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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