Phenotyping variability and detecting informative SSR and EST-SSR markers for malt and straw quality characteristics in a diverse panel of wild barley (H. spontaneum) under rainfed and irrigated conditions
Hooman Shirvani, Ali Ashraf Mehrabi, Mohsen Farshadfar, Hooshmand Safari, Ali Arminian, Foad Fatehi
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引用次数: 0
Abstract
Hordeum spontaneum Koch, the progenitor of cultivated barley, shares the same number of chromosomes as cultivated barley and can be easily crossed without any biological barriers. This study focuses on a collection of H. spontaneum obtained from natural habitats in the Zagros basin, Iran. This collection demonstrates a wide range of variations in seed quality, which were analyzed based on various parameters such as malting efficiency, cold and hot water extract, brix, reducing sugar, and color intensity. Additionally, the nutritional characteristics of the straw produced by each genotype of this germplasm were evaluated under rainfed and irrigated conditions, including crude protein (%CP), crude fiber (%CF), acid detergent fiber (%ADF), dry matter digestibility (%DMD), metabolism energy (ME), digestible energy (DE), and total digestible nutrient (TNT) characteristics. The genetic structure of the population was assessed using 97 different alleles amplified for 35 simple sequence repeat (SSR) and expressed sequence tag (EST)-derived SSR markers. The analysis revealed the presence of seven distinct sub-populations within this diverse panel of wild barley. Marker-trait association analysis identified the markers BeMAG0323 and GBM1461 as highly informative loci for the seed quality traits under both conditions. The markers SCSSR15864 and BMAC0154 were found to have the most significant association with the quality characteristics of the straw. This study is the first to establish a connection between straw traits and seed quality in barley by utilizing SSR and EST-SSR markers. By introducing new plant material that has been thoroughly studied for its economic qualitative characteristics in terms of seed and straw of barley, this research serves as a pre-breeding effort to enhance the quality of barley cultivars and to utilize the informative markers introduced here for future genomic selection programs.
期刊介绍:
Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation.
Areas of particular interest include:
-crop evolution
-domestication
-crop-weed relationships
-related wild species
-history of cultivated plants including palaeoethnobotany.
Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources.
Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany.
All contributions are in the English language and are subject to peer reviewing.