Unraveling the Molecular Mechanism of Lignin Accumulation in Stipule Thorns of Zanthoxylum bungeanum: Insights from Transcriptomic and Targeted Metabolomic Analysis

IF 3.9 3区 生物学 Q1 PLANT SCIENCES
Weilong Gao, Ling Zhou, Hao Zhong, Yuan Meng, Changle Li, Jianxin Wang, Jia Yao, Yulin Liu
{"title":"Unraveling the Molecular Mechanism of Lignin Accumulation in Stipule Thorns of Zanthoxylum bungeanum: Insights from Transcriptomic and Targeted Metabolomic Analysis","authors":"Weilong Gao, Ling Zhou, Hao Zhong, Yuan Meng, Changle Li, Jianxin Wang, Jia Yao, Yulin Liu","doi":"10.1007/s00344-024-11410-y","DOIUrl":null,"url":null,"abstract":"<p><i>Zanthoxylum bungeanum</i> (<i>Zb</i>), an economically important tree, is widely cultivated in China. However, its abundant and intricate thorns pose challenges in management and harvesting, thereby reducing its economic benefits. Although the origin and formation mechanism of stipule thorns in <i>Zb</i> remain unclear, it is hypothesized that thorn hardening may be associated with lignin synthesis and accumulation. In this study, we utilized histologic, transcriptomic, and metabolomic analysis methods with stipule thorns at five distinct developmental stages (25 days, 40 days, 55 days, 70 days, and 80 days after flowering) to investigate the mechanisms underlying lignin accumulation and synthesis. Our findings revealed that guaiacyl and syringyl lignin were present in the stipule thorns of <i>Zb</i>. Lignification occurs from the top to bottom and from the outside to inside. Through a weighted gene co-expression network analysis and construction of a gene regulation network, we identified 20 genes significantly involved in lignin synthesis and metabolism including 10 structural genes and 9 transcription factors such as <i>MYB</i>, <i>bHLH</i>, <i>WRKY</i>, and <i>NAC</i>. Notably, our target gene prediction results of hub genes indicated that four <i>NAC</i> genes play a critical role in lignin synthesis. Furthermore, we predicted a possible NAC-MYB model gene-regulatory network. This research provides novel insights into the synthesis of lignin in <i>Zb</i>, while offering a molecular foundation for breeding varieties of thornless or soft-thorned <i>Zb</i>.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00344-024-11410-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Zanthoxylum bungeanum (Zb), an economically important tree, is widely cultivated in China. However, its abundant and intricate thorns pose challenges in management and harvesting, thereby reducing its economic benefits. Although the origin and formation mechanism of stipule thorns in Zb remain unclear, it is hypothesized that thorn hardening may be associated with lignin synthesis and accumulation. In this study, we utilized histologic, transcriptomic, and metabolomic analysis methods with stipule thorns at five distinct developmental stages (25 days, 40 days, 55 days, 70 days, and 80 days after flowering) to investigate the mechanisms underlying lignin accumulation and synthesis. Our findings revealed that guaiacyl and syringyl lignin were present in the stipule thorns of Zb. Lignification occurs from the top to bottom and from the outside to inside. Through a weighted gene co-expression network analysis and construction of a gene regulation network, we identified 20 genes significantly involved in lignin synthesis and metabolism including 10 structural genes and 9 transcription factors such as MYB, bHLH, WRKY, and NAC. Notably, our target gene prediction results of hub genes indicated that four NAC genes play a critical role in lignin synthesis. Furthermore, we predicted a possible NAC-MYB model gene-regulatory network. This research provides novel insights into the synthesis of lignin in Zb, while offering a molecular foundation for breeding varieties of thornless or soft-thorned Zb.

Abstract Image

揭示 Zanthoxylum bungeanum 托叶荆棘木质素积累的分子机制:转录组和靶向代谢组分析的启示
Zanthoxylum bungeanum(Zb)是一种具有重要经济价值的树种,在中国被广泛种植。然而,其丰富而复杂的刺给管理和采收带来了挑战,从而降低了其经济效益。虽然浙贝母托叶刺的起源和形成机制尚不清楚,但有假设认为刺的硬化可能与木质素的合成和积累有关。在本研究中,我们利用组织学、转录组和代谢组分析方法,对五个不同发育阶段(花后 25 天、40 天、55 天、70 天和 80 天)的托叶刺进行了研究,以探讨木质素积累和合成的机制。我们的研究结果表明,Zb 的托叶刺中存在愈创木基和丁香基木质素。木质化是由上至下、由外至内发生的。通过加权基因共表达网络分析和基因调控网络构建,我们发现了20个显著参与木质素合成和代谢的基因,包括10个结构基因和9个转录因子,如MYB、bHLH、WRKY和NAC。值得注意的是,我们对枢纽基因的靶基因预测结果表明,4 个 NAC 基因在木质素合成中起着关键作用。此外,我们还预测了一个可能的 NAC-MYB 模型基因调控网络。这项研究为枸骨木质素的合成提供了新的见解,同时也为培育无刺或软刺枸骨品种提供了分子基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信