Hsa_Circ_002144 Promotes Glycolysis and Immune Escape of Breast Cancer Through miR-326/PKM Axis.

IF 2.4 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Cancer Biotherapy and Radiopharmaceuticals Pub Date : 2024-07-04 DOI:10.1089/cbr.2024.0009

Yong Yang, Tianhao Xie, Peng Gao, Weijun Han, Yuhong Liu, Yanmei Wang

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引用次数: 0

Abstract

Background: Breast cancer is a leading cause of cancer-related deaths in women worldwide, posing a significant threat to female health. Therefore, it is crucial to search for new therapeutic targets and prognostic biomarkers for breast cancer patients. Method: Bioinformatics analysis, quantitative real-time PCR (qRT-PCR), and fluorescence in situ hybridization (FISH) were employed to investigate the expression of hsa_circ_002144 in breast cancer. Transwell assay, Western blotting, and cell viability assay were utilized to assess the impact of hsa_circ_002144 on the proliferation, migration, and invasion of breast cancer cells. Additionally, a mouse model was established to validate its functionality. Flow cytometry, WB analysis, enzyme-linked immunosorbent assay (ELISA), qRT-PCR, exosomes isolation, and co-culture system were employed to elucidate the molecular mechanism underlying macrophage polarization. Result: we have discovered for the first time that hsa_circ_002144 is highly expressed in breast cancer. It affected tumor growth and metastasis and could influence macrophage polarization through the glycolytic pathway. Conclusion: This finding provides a new direction for breast cancer treatment and prognosis assessment.

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Hsa_Circ_002144通过miR-326/PKM轴促进糖酵解和乳腺癌的免疫逃逸

背景：乳腺癌是全球女性因癌症死亡的主要原因之一，对女性健康构成重大威胁。因此，为乳腺癌患者寻找新的治疗靶点和预后生物标志物至关重要。研究方法采用生物信息学分析、定量实时 PCR（qRT-PCR）和荧光原位杂交（FISH）等方法研究 hsa_circ_002144 在乳腺癌中的表达。此外，还利用Transwell试验、Western印迹和细胞活力试验评估了hsa_circ_002144对乳腺癌细胞增殖、迁移和侵袭的影响。此外，还建立了一个小鼠模型来验证其功能。采用流式细胞术、WB 分析、酶联免疫吸附试验（ELISA）、qRT-PCR、外泌体分离和共培养系统来阐明巨噬细胞极化的分子机制。结果：我们首次发现了 hsa_circ_002144 在乳腺癌中的高表达。它影响肿瘤的生长和转移，并能通过糖酵解途径影响巨噬细胞的极化。结论这一发现为乳腺癌的治疗和预后评估提供了新的方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Biotherapy and Radiopharmaceuticals 医学-核医学

CiteScore

7.80

自引率

2.90%

发文量

审稿时长

3 months

期刊介绍： Cancer Biotherapy and Radiopharmaceuticals is the established peer-reviewed journal, with over 25 years of cutting-edge content on innovative therapeutic investigations to ultimately improve cancer management. It is the only journal with the specific focus of cancer biotherapy and is inclusive of monoclonal antibodies, cytokine therapy, cancer gene therapy, cell-based therapies, and other forms of immunotherapies. The Journal includes extensive reporting on advancements in radioimmunotherapy, and the use of radiopharmaceuticals and radiolabeled peptides for the development of new cancer treatments.