Comparative genomics of Xanthomonas cucurbitae isolates collected from Midwestern United States pumpkin fields

IF 2.3 3区农林科学 Q1 AGRONOMY

Plant Pathology Pub Date : 2024-06-29 DOI:10.1111/ppa.13965

Rikky Rai, Kayla M. Vittore, Julius Pasion, Maria L. Malvino, Jonathan D. Mason, Qiong Liu, Salisu Sulley, Mohammad Babadoost, Julian M. Catchen, Sarah R. Hind

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Abstract

Bacterial spot disease of cucurbits, caused by Xanthomonas cucurbitae, is a major problem in cucurbit‐growing areas worldwide. In the Midwestern region of the United States, pumpkin and squash fields can have greater than 90% infected fruits, leading to high yield losses. While reference genomes are available for this bacterial species, the genetic diversity between different strains and populations is unknown. After performing restriction‐site associated DNA sequencing (RAD‐seq) analysis of X. cucurbitae isolates collected from the Midwestern region, we selected five representative isolates for further characterization, which included whole‐genome sequencing and in vitro enzyme and in planta virulence assays. Our results suggest that minimal genetic diversity exists between these isolates, and that the isolates have differential virulence on different cucurbit host plants. This study contributes to our understanding of X. cucurbitae population dynamics in the Midwestern region and may assist with developing additional management strategies for controlling bacterial spot disease of cucurbits.

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从美国中西部南瓜田采集的葫芦黄单胞菌分离物的比较基因组学研究

由葫芦黄单胞菌（Xanthomonas cucurbitae）引起的葫芦细菌性斑点病是全世界葫芦种植区的一个主要问题。在美国中西部地区，南瓜和南瓜田里 90% 以上的果实都会受到感染，导致高产损失。虽然这种细菌有参考基因组，但不同菌株和种群之间的遗传多样性尚不清楚。在对从中西部地区收集到的 X. cucurbitae 分离物进行限制性位点相关 DNA 测序（RAD-seq）分析后，我们选择了五个具有代表性的分离物进行进一步鉴定，包括全基因组测序、体外酶和植物毒力测定。我们的研究结果表明，这些分离株之间的遗传多样性极小，而且这些分离株对不同的葫芦科寄主植物具有不同的毒力。这项研究有助于我们了解 X. cucurbitae 在中西部地区的种群动态，并有助于制定更多的管理策略来控制葫芦的细菌性斑点病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Pathology 生物-农艺学

CiteScore

5.60

自引率

7.40%

发文量

147

审稿时长

3 months

期刊介绍： This international journal, owned and edited by the British Society for Plant Pathology, covers all aspects of plant pathology and reaches subscribers in 80 countries. Top quality original research papers and critical reviews from around the world cover: diseases of temperate and tropical plants caused by fungi, bacteria, viruses, phytoplasmas and nematodes; physiological, biochemical, molecular, ecological, genetic and economic aspects of plant pathology; disease epidemiology and modelling; disease appraisal and crop loss assessment; and plant disease control and disease-related crop management.