PCR compatible miniprep DNA isolation in rice using microwave and dry bath based heating devices

Abstract

An effective and fast miniprep DNA protocol is on demand among plant molecular biologist where two moderns heating devices (microwave and dry bath) may be adopted in place of conventional water bath which is time consuming and sometimes result in microbial contamination. Though most of the present-day modern laboratories are equipped with these two recent heating devices, very limited reports are available using these two devices for isolation of high-quality plant genomic DNA with desirable quantity. Addressing this demand, in present study an earlier adopted DNA isolation protocol was re-standardized on different rice tissue lines seedlings, leaves, stem and root with the inclusion of microwave and dry bath particularly for the duration and applied temperature during heating followed by comparing with popularly used protocol for yield and quality of the isolated genomic DNA through standard statistical analysis. Pair- wise t test unable to detect any significant differences (for both quality and quantity) between the earlier standardized protocol with the two newly standardized protocol (adopted with microwave and dry bath) for the isolated genomic DNA from the different plant tissues included. The isolated genomic DNA was amplified through PCR followed by resolving through agarose gel electrophoresis to confirm their PCR compatibility.