{"title":"A multiplexing system for quantifying oxygen fractionation factors in closed chambers","authors":"Clémence Paul, Clément Piel, Joana Sauze, Olivier Jossoud, Arnaud Dapoigny, Daniele Romanini, Frédérique Prié, Sébastien Devidal, Roxanne Jacob, Alexandru Milcu, Amaëlle Landais","doi":"10.5194/egusphere-2024-1755","DOIUrl":null,"url":null,"abstract":"<strong>Abstract.</strong> The study of isotopic ratios of atmospheric oxygen in fossilized air trapped in ice core bubbles provides information on variations in the hydrological cycle at low latitudes and productivity in the past. However, to refine these interpretations, it is necessary to better quantify fractionation of oxygen in the biological processes such as photosynthesis and respiration. We set up a system of closed biological chambers in which we studied the evolution of elemental and isotopic composition of O<sub>2</sub> due to biological processes. To easily replicate experiments, we developed a multiplexing system which we describe here. We compared measurements of elemental and isotopic composition of O<sub>2</sub> using two different measurement techniques: optical spectrometry (Optical-Feedback Cavity- Enhanced Absorption Spectroscopy, i.e. OF-CEAS technique), which enables higher temporal resolution and continuous data collection and isotopic ratio mass spectrometry (IRMS) with a flanged air recovery system, thus validating the data analysis conducted through the OF-CEAS technique. As a first application, we investigated isotopic discrimination during respiration and photosynthesis. We conducted a 5-day experiment using maize (<em>Zea mays</em> L.) as model species. The <sup>18</sup>O discrimination value for maize during dark plant respiration was determined as - 17.8 ± 0.9 ‰ by IRMS and - 16.1 ± 1.1 ‰ by optical spectrometer. We also found a value attributed to the isotopic discrimination of terrestrial photosynthesis equal to + 3.2 ± 2.6 ‰ by IRMS and + 6.7 ± 3.8 ‰ by optical spectrometer. These findings were consistent with a previous study by Paul et al. (2023).","PeriodicalId":48742,"journal":{"name":"Geoscientific Instrumentation Methods and Data Systems","volume":"158 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Geoscientific Instrumentation Methods and Data Systems","FirstCategoryId":"89","ListUrlMain":"https://doi.org/10.5194/egusphere-2024-1755","RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GEOSCIENCES, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract. The study of isotopic ratios of atmospheric oxygen in fossilized air trapped in ice core bubbles provides information on variations in the hydrological cycle at low latitudes and productivity in the past. However, to refine these interpretations, it is necessary to better quantify fractionation of oxygen in the biological processes such as photosynthesis and respiration. We set up a system of closed biological chambers in which we studied the evolution of elemental and isotopic composition of O2 due to biological processes. To easily replicate experiments, we developed a multiplexing system which we describe here. We compared measurements of elemental and isotopic composition of O2 using two different measurement techniques: optical spectrometry (Optical-Feedback Cavity- Enhanced Absorption Spectroscopy, i.e. OF-CEAS technique), which enables higher temporal resolution and continuous data collection and isotopic ratio mass spectrometry (IRMS) with a flanged air recovery system, thus validating the data analysis conducted through the OF-CEAS technique. As a first application, we investigated isotopic discrimination during respiration and photosynthesis. We conducted a 5-day experiment using maize (Zea mays L.) as model species. The 18O discrimination value for maize during dark plant respiration was determined as - 17.8 ± 0.9 ‰ by IRMS and - 16.1 ± 1.1 ‰ by optical spectrometer. We also found a value attributed to the isotopic discrimination of terrestrial photosynthesis equal to + 3.2 ± 2.6 ‰ by IRMS and + 6.7 ± 3.8 ‰ by optical spectrometer. These findings were consistent with a previous study by Paul et al. (2023).
期刊介绍:
Geoscientific Instrumentation, Methods and Data Systems (GI) is an open-access interdisciplinary electronic journal for swift publication of original articles and short communications in the area of geoscientific instruments. It covers three main areas: (i) atmospheric and geospace sciences, (ii) earth science, and (iii) ocean science. A unique feature of the journal is the emphasis on synergy between science and technology that facilitates advances in GI. These advances include but are not limited to the following:
concepts, design, and description of instrumentation and data systems;
retrieval techniques of scientific products from measurements;
calibration and data quality assessment;
uncertainty in measurements;
newly developed and planned research platforms and community instrumentation capabilities;
major national and international field campaigns and observational research programs;
new observational strategies to address societal needs in areas such as monitoring climate change and preventing natural disasters;
networking of instruments for enhancing high temporal and spatial resolution of observations.
GI has an innovative two-stage publication process involving the scientific discussion forum Geoscientific Instrumentation, Methods and Data Systems Discussions (GID), which has been designed to do the following:
foster scientific discussion;
maximize the effectiveness and transparency of scientific quality assurance;
enable rapid publication;
make scientific publications freely accessible.