Recovery of bluetongue virus serogroup from sera collected for a serological survey from apparently healthy cattle, from the Sudan.

E M Abu Elzein
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引用次数: 8

Abstract

Virus of the bluetongue (BT) serogroup was recovered from 11% of cattle sera collected from apparently healthy animals in Khartoum Province for the sole purpose of screening for BT antibodies. Since these sera did not contain BT antibodies, the donor cattle could have been scored as BT free in the serological survey. Virus was initially isolated in chicken embryos inoculated intravascularly, and was further adapted to Vero cell cultures. Isolates were identified as belonging to the BT serogroup using the agar gel immunodiffusion (AGID) and complement fixation (CF) tests. The results indicated that cattle in the Sudan could harbour BT virus without showing symptoms of the disease. Such an observation necessitates further work to clarify the role of cattle in the epidemiology of BT in the Sudan.

从苏丹为进行血清学调查而收集的牛血清中恢复蓝舌病病毒血清组。
在喀土穆省,仅为筛查蓝舌病抗体而从表面健康的动物身上采集的牛血清中提取了11%的蓝舌病血清组病毒。由于这些血清不含BT抗体,供体牛在血清学调查中可以被标记为无BT。病毒最初是从接种于血管内的鸡胚中分离出来的,并进一步适应于Vero细胞培养。通过琼脂凝胶免疫扩散(AGID)和补体固定(CF)试验鉴定分离株属于BT血清组。结果表明,苏丹的牛可以携带BT病毒而不表现出该疾病的症状。这样的观察结果需要进一步的工作来澄清牛在苏丹BT流行病学中的作用。
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