Identification of host immune-related biomarkers in active tuberculosis: A comprehensive analysis of differentially expressed genes

IF 2.8 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2024-06-28 DOI:10.1016/j.tube.2024.102538

Alisha Ansari , Gajendra Pratap Singh , Mamtesh Singh , Harpreet Singh

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引用次数: 0

Abstract

Tuberculosis (TB) is a serious public health issue in India. Numerous molecular mechanisms and immunological responses play significant roles in the pathogenesis of tuberculosis. This study aimed to identify host immune-related biomarkers that are significantly differentially expressed in active TB and that play a vital role in disease progression. The methodology employed in this study included data collection, pre-processing, analysis, and interpretation of the results. Six microarray datasets were used to identify differentially expressed genes (DEGs), and only the common DEGs were used for further downstream analysis, such as hub gene identification, gene ontology, pathway enrichment analysis, and drug-gene interaction analysis. The study identified 1728 DEGs, including 906 upregulated and 822 downregulated genes. Five hub genes were identified that were: STAT1, GBP5, GBP1, FCGR1A, and BATF2. Gene ontology and pathway enrichment revealed that most of the genes were involved in interferon-gamma signaling. In addition, through drug-gene interactions, known drugs have been identified for STAT1, FCGR1A and GBP1. The findings of this study may contribute to early detection and treatment of active TB.

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活动性结核病宿主免疫相关生物标志物的鉴定：全面分析差异表达基因

结核病（TB）是印度一个严重的公共卫生问题。许多分子机制和免疫反应在结核病的发病机制中发挥着重要作用。本研究旨在确定在活动性结核病中显著差异表达的宿主免疫相关生物标记物，这些标记物在疾病进展中发挥着重要作用。本研究采用的方法包括数据收集、预处理、分析和结果解释。研究使用了六个微阵列数据集来鉴定差异表达基因（DEGs），并仅使用常见的 DEGs 进行进一步的下游分析，如中心基因鉴定、基因本体论、通路富集分析和药物基因相互作用分析。研究发现了 1728 个 DEGs，包括 906 个上调基因和 822 个下调基因。确定了五个枢纽基因，分别是STAT1、GBP5、GBP1、FCGR1A 和 BATF2。基因本体论和通路富集显示，大部分基因参与了干扰素-γ 信号转导。此外，通过药物与基因的相互作用，发现了 STAT1、FCGR1A 和 GBP1 的已知药物。这项研究的发现可能有助于活动性肺结核的早期检测和治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.