Cellular distribution of AQP1, AQP2, and AQP3 in ureter and urinary bladder of goats in different seasons

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Small Ruminant Research Pub Date : 2024-07-01 DOI:10.1016/j.smallrumres.2024.107308

Smriti Shukla , Aditya D. Deshpande , Ashutosh , Sachinandan De

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引用次数: 0

Abstract

Aquaporins (AQP) are transmembrane proteins that function as channels for water molecules' movement. It is conserved across bacteria, plants and animals. It helps in thermoregulation during extreme climatic conditions. So, this study focuses on the expression of AQP1, AQP2 and AQP3 in the ureter and bladder tissues of goat (Capra hircus) during winter, spring and summer seasons. The study was conducted on goats (n=30) to investigate the expression of AQP (water channels) in ureter and urinary bladder and metabolic and hormonal influence by aquaporins. mRNAs were isolated from ureter and urinary bladder tissues and cDNA were synthesized for RT-PCR expression studies. Biochemical parameters were studied in the blood samples. AQP1 mRNA expression was non-significant in goat ureters but considerably greater (p<0.05) in the urine bladder during summer compared to winter. During the summer, both ureter and urinary bladder tissue samples showed increased expression of AQP2 and AQP3. Aquaporins 3 had significantly greater (p<0.05) mRNA expression in the ureter and urine bladder than Aquaporins 1 and 2 throughout the summer season. The significant (p<0.05) increase in physiological responses, haematological counts and blood biochemical components seen in goats throughout the summer season. Finally, it concludes that AQP and their role in thermotolerance and direct association with effective water balance mechanisms in the body via expression of AQP in different extreme climatic conditions.

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不同季节山羊输尿管和膀胱中 AQP1、AQP2 和 AQP3 的细胞分布情况

水汽蛋白（AQP）是一种跨膜蛋白，具有水分子移动通道的功能。它在细菌、植物和动物中都是保守的。它有助于在极端气候条件下进行体温调节。因此，本研究重点关注冬、春、夏三季山羊（Capra hircus）输尿管和膀胱组织中 AQP1、AQP2 和 AQP3 的表达情况。研究以山羊（n=30）为对象，调查输尿管和膀胱中 AQP（水通道）的表达以及水通道蛋白对代谢和激素的影响。对血液样本中的生化指标进行了研究。与冬季相比，夏季山羊输尿管中的 AQP1 mRNA 表达量并不显著，但膀胱中的表达量却大大增加（p<0.05）。在夏季，输尿管和膀胱组织样本中 AQP2 和 AQP3 的表达量都有所增加。在整个夏季，输尿管和膀胱中 Aquaporins 3 的 mRNA 表达量明显高于 Aquaporins 1 和 2（p<0.05）。在整个夏季，山羊的生理反应、血细胞计数和血液生化成分都明显增加（p<0.05）。最后，研究得出结论：在不同的极端气候条件下，AQP 及其在耐热性中的作用以及通过 AQP 的表达与体内有效的水分平衡机制直接相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Small Ruminant Research 农林科学-奶制品与动物科学

CiteScore

3.10

自引率

11.10%

发文量

210

审稿时长

12.5 weeks

期刊介绍： Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.