Vanadium haloperoxidases as noncanonical terpene synthases.

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-04-17 DOI:10.1016/bs.mie.2024.03.024
Jackson T Baumgartner, Lia I Lozano Salazar, Lukas A Varga, Gabriel H Lefebre, Shaun M K McKinnie
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引用次数: 0

Abstract

Vanadium-dependent haloperoxidases (VHPOs) are a unique family of enzymes that utilize vanadate, an aqueous halide ion, and hydrogen peroxide to produce an electrophilic halogen species that can be incorporated into electron rich organic substrates. This halogen species can react with terpene substrates and trigger halonium-induced cyclization in a manner reminiscent of class II terpene synthases. While not all VHPOs act in this capacity, several notable examples from algal and actinobacterial species have been characterized to catalyze regio- and enantioselective reactions on terpene and meroterpenoid substrates, resulting in complex halogenated cyclic terpenes through the action of single enzyme. In this article, we describe the expression, purification, and chemical assays of NapH4, a difficult to express characterized VHPO that catalyzes the chloronium-induced cyclization of its meroterpenoid substrate.

作为非典型萜烯合成酶的钒卤过氧化物酶
依赖钒的卤代氧化酶(VHPOs)是一个独特的酶家族,它利用钒酸盐、一种含水卤离子和过氧化氢产生一种亲电卤素,这种卤素可以与富含电子的有机底物结合。这种卤素可与萜烯底物发生反应,并以类似于第二类萜烯合成酶的方式引发卤素诱导的环化反应。虽然并不是所有的 VHPOs 都具有这种功能,但藻类和放线菌物种中的几个著名例子已被证实可以催化萜烯和经萜烯底物的区域和对映体选择性反应,通过单酶的作用产生复杂的卤代环萜烯。在这篇文章中,我们描述了 NapH4 的表达、纯化和化学分析。NapH4 是一种难以表达的 VHPO,其特征是催化其经萜类底物的氯诱导环化反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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