Quantification of Rhodojaponin II and Rhodojaponin III in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.

IF 1.5 4区化学 Q3 CHEMISTRY, ANALYTICAL

International Journal of Analytical Chemistry Pub Date : 2024-06-18 eCollection Date: 2024-01-01 DOI:10.1155/2024/6386570

Cheng Sun, Wanhang Wang, Xi Bao, Dizhong Chen, Shenshen Mei, Jianshe Ma, Xiajuan Jiang

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Abstract

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to determine the concentrations of Rhodojaponin II and Rhodojaponin III in rat plasma, and their pharmacokinetic profiles were investigated. A UPLC HSS T3 (2.1 mm × 50 mm, 1.8 μm) chromatographic column was employed at a temperature of 40°C. The mobile phase consisted of acetonitrile-0.1% formic acid in water, and a gradient elution method with an elution time of 6 min and flow rate of 0.4 mL/min was utilized for analysis purposes. Methodological investigations were conducted accordingly. The plasma concentrations of Rhodojaponin II and Rhodojaponin III exhibited excellent linearity within the range of 2 ng/mL-1250 ng/mL. Moreover, both intraday and interday precision were below 15%, while accuracy ranged from 88% to 115%. Additionally, matrix effect fell within the range of 90%-110%, and recoveries ranged from 78% to 87%. These results comply with relevant regulations for drug analysis in biological samples. Therefore, this method is deemed suitable for quantifying Rhodojaponin II and Rhodojaponin III levels in rats.

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超高效液相色谱-串联质谱法定量检测大鼠血浆中的红豆皂苷 II 和红豆皂苷 III

建立了超高效液相色谱-串联质谱（UPLC-MS/MS）测定大鼠血浆中黄连皂苷II和黄连皂苷III浓度的方法，并考察了它们的药代动力学特征。采用UPLC HSS T3 (2.1 mm × 50 mm, 1.8 μm)色谱柱，温度为40 ℃。以乙腈-0.1%甲酸水溶液为流动相，采用梯度洗脱法进行分析，洗脱时间为 6 分钟，流速为 0.4 mL/min。进行了相应的方法学研究。Rhodojaponin II 和 Rhodojaponin III 的血浆浓度在 2 ng/mL-1250 ng/mL 范围内呈良好的线性关系。此外，日内和日间精密度均低于 15%，准确度在 88% 至 115% 之间。此外，基质效应在 90%-110% 之间，回收率在 78%-87% 之间。这些结果符合生物样本中药物分析的相关规定。因此，该方法适用于大鼠体内红豆皂苷 II 和红豆皂苷 III 的定量分析。

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来源期刊

International Journal of Analytical Chemistry CHEMISTRY, ANALYTICAL-

CiteScore

3.10

自引率

5.60%

发文量

117

期刊介绍： International Journal of Analytical Chemistry publishes original research articles that report new experimental results and methods, especially in relation to important analytes, difficult matrices, and topical samples. Investigations may be fundamental, or else related to specific applications; examples being biological, environmental and food testing, and analysis in chemical synthesis and materials processing. As well as original research, the International Journal of Analytical Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.