Genome-wide DNA methylation profiles regulate distinct heat stress response in zebu (Bos indicus) and crossbred (Bos indicus × Bos taurus) cattle

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-06-25 DOI:10.1016/j.cstres.2024.06.005

Basavaraj Sajjanar , Mohd Tanzeel Aalam , Owais Khan , Sujoy K Dhara , Jyotirmoy Ghosh , Ravi Kumar Gandham , Praveen K Gupta , Pallab Chaudhuri , Triveni Dutt , Gyanendra Singh , Bishnu Prasad Mishra

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引用次数: 0

Abstract

Epigenetic variations result from long-term adaptation to environmental factors. The Bos indicus (zebu) adapted to tropical conditions, whereas Bos taurus adapted to temperate conditions; hence native zebu cattle and its crossbred (B indicus × B taurus) show differences in responses to heat stress. The present study evaluated genome-wide DNA methylation profiles of these two breeds of cattle that may explain distinct heat stress responses. Physiological responses to heat stress and estimated values of Iberia heat tolerance coefficient and Benezra's coefficient of adaptability revealed better relative thermotolerance of Hariana compared to the Vrindavani cattle. Genome-wide DNA methylation patterns were different for Hariana and Vrindavani cattle. The comparison between breeds indicated the presence of 4599 significant differentially methylated CpGs with 756 hypermethylated and 3845 hypomethylated in Hariana compared to the Vrindavani cattle. Further, we found 79 genes that showed both differential methylation and differential expression that are involved in cellular stress response functions. Differential methylations in the microRNA coding sequences also revealed their functions in heat stress responses. Taken together, epigenetic differences represent the potential regulation of long-term adaptation of Hariana (B indicus) cattle to the tropical environment and relative thermotolerance.

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全基因组 DNA 甲基化图谱调控斑马牛（Bos indicus）和杂交牛（Bos indicus X Bos taurus）不同的热应激反应。

表观遗传变异源于对环境因素的长期适应。斑马牛（Bos indicus）适应热带环境，而牛适应温带环境，因此本地斑马牛及其杂交品种（B indicus X B taurus）对热应激的反应存在差异。本研究评估了这两个牛种的全基因组 DNA 甲基化图谱，这可能解释了不同的热应激反应。对热应激的生理反应以及伊比利亚耐热系数（HTC）和贝内兹拉适应性系数（BCA）的估计值表明，与弗林达瓦尼牛相比，哈里亚纳牛的相对耐热性更好。哈里亚纳牛和弗林达瓦尼牛的全基因组 DNA 甲基化模式不同。品种间的比较表明，Hariana 牛与 Vrindavani 牛相比，存在 4599 个显著的差异甲基化 CpGs（DMC），其中 756 个为高甲基化，3845 个为低甲基化。此外，我们还发现有 79 个基因同时表现出不同的甲基化和不同的表达，这些基因涉及细胞应激反应功能。微小核糖核酸（microRNA）编码序列的甲基化差异也揭示了它们在热应激反应中的功能。综上所述，表观遗传学差异代表了哈里亚纳牛（B indicus）对热带环境和相对耐热性的长胚期适应性的潜在调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567