Spirulina supplement and exercise training affect lipid droplets-related genes expression in visceral adipose tissue.

IF 1.9 Q3 CHEMISTRY, MEDICINAL

Avicenna Journal of Phytomedicine Pub Date : 2024-01-01 DOI:10.22038/AJP.2023.22915

Fariba Shahandeh, Rozita Fathi, Khadijeh Nasiri

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引用次数: 0

Abstract

Objective: Disruption of lipid droplets (LDs) is associated with many metabolic diseases. Spirulina, as a natural bioactive dietary supplement, along with exercise training, may improve lipid metabolism; however, their effects on LDs-regulated genes in visceral adipose tissue are still unclear. This study aimed to investigate the effects of six-week Spirulina supplementation along with exercise training on LDs regulating gene expression.

Materials and methods: Fifty-six male Wistar rats were divided into six groups: saline (control), control+Spirulina (Spirulina), aerobic interval training (AIT), AIT+ Spirulina (AIT+Spirulina), resistance training and resistance+ Spirulina. The supplement groups consumed 500 mg/kg Spirulina five days per week. The training groups performed AIT (5 times per week) and resistance training (3 times per week) for 6 weeks. LDs regulating genes expression in visceral adipose tissue (Zw10, Bscl2, DFCP1, Rab18, Syntaxin 18, Acsl3, and Plin2) was analyzed by real-time PCR.

Results: Spirulina and exercise training had no significant effects on the gene expression of Syntaxin18 (p=0.69) and DFCP1 (p=0. 84), ACSL3 (p=0.98), or BSCL2 (p=0.58). In addition, Spirulina was found to significantly attenuate the expression of Plin2 (p=0.01) and Rab18 (p=0.01) genes compared to the control, AIT, and resistance training groups. However, Plin2 gene expression was higher in the resistance training than the AIT. Furthermore, Spirulina decreased ZW10 (p=0.03) gene expression in visceral adipose tissue compared to the control, AIT, and resistance training groups. Unexpectedly, Spirulina supplementation decreased the expression of these genes even more when taken without exercise training.

Conclusion: Spirulina supplementation and exercise training have significant effects on LDs-regulated genes in visceral adipose tissue.

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螺旋藻补充剂和运动训练会影响内脏脂肪组织中脂滴相关基因的表达。

目的：脂滴（LDs）的破坏与许多代谢性疾病有关。螺旋藻作为一种天然生物活性膳食补充剂，配合运动训练可改善脂质代谢；然而，它们对内脏脂肪组织中LDs调控基因的影响仍不清楚。本研究旨在探讨在补充六周螺旋藻的同时进行运动训练对 LDs 调控基因表达的影响：将 56 只雄性 Wistar 大鼠分为 6 组：生理盐水组（对照组）、对照组+螺旋藻组（螺旋藻组）、有氧间歇训练组（AIT 组）、AIT+螺旋藻组（AIT+螺旋藻组）、阻力训练组和阻力+螺旋藻组。补充组每周五天摄入 500 毫克/千克螺旋藻。训练组进行 AIT（每周 5 次）和阻力训练（每周 3 次），为期 6 周。通过实时 PCR 分析了内脏脂肪组织中调节基因表达的 LDs（Zw10、Bscl2、DFCP1、Rab18、Syntaxin 18、Acsl3 和 Plin2）：结果：螺旋藻和运动训练对 Syntaxin18（P=0.69）、DFCP1（P=0.84）、ACSL3（P=0.98）或 BSCL2（P=0.58）的基因表达没有显著影响。此外，与对照组、AIT 组和阻力训练组相比，螺旋藻能显著降低 Plin2（p=0.01）和 Rab18（p=0.01）基因的表达。然而，阻力训练组的 Plin2 基因表达高于 AIT 组。此外，与对照组、AIT 组和阻力训练组相比，螺旋藻降低了内脏脂肪组织中 ZW10（p=0.03）基因的表达。意想不到的是，在不进行运动训练的情况下，补充螺旋藻会进一步降低这些基因的表达：结论：补充螺旋藻和运动训练对内脏脂肪组织中的低密度脂蛋白调节基因有显著影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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