Protective effect of ellagic acid against high-glucose-induced injury in human umbilical venous endothelial cells.

IF 1.9 Q3 CHEMISTRY, MEDICINAL
Somayeh Sheikh, Hesam Dehghani, Hamid Reza Kazerani
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引用次数: 0

Abstract

Objective: There is escalating evidence suggesting the beneficial effects of ellagic acid (EA) on the cardiovascular system. The aim of the present study was to investigate the protective effect of EA in human umbilical vein endothelial cells (HUVECs) against high glucose (HG)- induced endothelial dysfunction and to study the potential roles of adropin and nitric oxide (NO) in this regard.

Materials and methods: The experimental groups consisted of normal and HG (30 mM, 48 hr)-treated HUVECs incubated without or with 5 or 10 μM of EA (6 groups of at least 6 replicates, each). The cell count and viability were studied. Moreover, the markers of the redox state, including malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase enzymes, and ferric reducing anti-oxidant power (FRAP), were assayed. The levels of adropin and eNOS gene expression were also studied using RT-qPCR.

Results: A high concentration of glucose reduced cell count and caused lipid peroxidation, reduced anti-oxidant capacity of the cells, decreased NO levels, and downregulated the expression of NOS3 (encoding eNOS) and ENHO (encoding adropin) genes. Ellagic acid reversed all these effects.

Conclusion: These results suggest a significant protective effect for EA against HG-induced injury in HUVECs. The improved redox state and upregulation of NOS3 and ENHO genes seem to play critical roles in this regard.

鞣花酸对高血糖诱导的人脐静脉内皮细胞损伤的保护作用。
目的:越来越多的证据表明鞣花酸(EA)对心血管系统有益。本研究旨在探讨鞣花酸在人脐静脉内皮细胞(HUVECs)中对高葡萄糖(HG)诱导的内皮功能障碍的保护作用,并研究阿糖腺苷和一氧化氮(NO)在这方面的潜在作用:实验组由正常和经 HG(30 mM,48 小时)处理的 HUVECs 组成,这些 HUVECs 不含或含有 5 或 10 μM 的 EA(6 组,每组至少 6 个重复)。研究了细胞数量和存活率。此外,还检测了氧化还原状态的标志物,包括丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶的活性以及铁还原抗氧化能力(FRAP)。此外,还使用 RT-qPCR 技术研究了腺嘌呤和 eNOS 基因的表达水平:结果:高浓度葡萄糖会减少细胞数量,导致脂质过氧化,降低细胞的抗氧化能力,降低 NO 水平,并下调 NOS3(编码 eNOS)和 ENHO(编码 adropin)基因的表达。鞣花酸可逆转所有这些影响:这些结果表明,EA 对 HG 诱导的 HUVEC 损伤有明显的保护作用。在这方面,氧化还原状态的改善以及 NOS3 和 ENHO 基因的上调似乎起着关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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