[Construction of a mouse model of type 2 diabetes induced by high fat diet alone and evaluation of pathological changes].

Q3 Medicine
生理学报 Pub Date : 2024-06-25
Chen-Ge Yu, Jing Huang, Yan Xi, En-Yin Lai, Su Chen, Nan Xu
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Abstract

The purpose of the present study was to investigate the modeling time of type 2 diabetes mellitus (T2DM) mouse model induced by high fat diet (HFD) alone and the effects of HFD on the pathology and function of organs related to glucose and lipid metabolism. C57BL/6 mice were fed with normal diet (NC group) or HFD (HFD group). The time of successful T2DM modeling was evaluated by measuring body weight, fasting blood glucose and glucose tolerance at time points of 0, 4, 8, 12, 16 and 20 weeks. The functional and pathological changes of glucose and lipid metabolism related organs were evaluated by detecting insulin tolerance, plasma lipid levels, vascular function, as well as HE staining of pancreas and liver. The results showed that compared with the NC group, the HFD group had significantly increased body weight after 8 weeks of HFD. After 16 weeks of HFD, the HFD group exhibited impaired fasting glucose tolerance. After 20 weeks of HFD, the HFD group mice reached diabetic state, showing impaired glucose tolerance and insulin resistance, islet volume reduction and vacuolar degeneration; Large number of lipid droplets appeared in liver cells, and the level of AMPK phosphorylation in liver tissue was significantly increased in the HFD groups, compared with the NC group; There was endothelial dependent diastolic dysfunction in the thoracic aorta of the HFD group; Compared with the NC group, the HFD group mice showed a significant increase in urinary protein levels. These results suggest that T2DM mouse model can be successfully established by HFD induction alone for 20 weeks. The model is characterized by insulin resistance, fatty liver, hyperlipidemia, vascular dysfunction, renal dysfunction and pathological changes of islet and liver cells, which are similar to those of T2DM patients. Therefore it can be used as an ideal animal model for T2DM research.

[建立单纯高脂饮食诱导的 2 型糖尿病小鼠模型并评估病理变化]。
本研究旨在探讨单纯高脂饮食(HFD)诱导的2型糖尿病(T2DM)小鼠模型的建模时间,以及HFD对糖、脂代谢相关器官的病理和功能的影响。C57BL/6 小鼠饲喂正常饮食(NC 组)或高脂饮食(HFD 组)。在 0、4、8、12、16 和 20 周的时间点测量体重、空腹血糖和糖耐量,评估 T2DM 成功建模的时间。通过检测胰岛素耐受性、血浆脂质水平、血管功能以及胰腺和肝脏的 HE 染色,评估糖脂代谢相关器官的功能和病理变化。结果显示,与NC组相比,HFD组在HFD 8周后体重明显增加。高密度脂蛋白饮食 16 周后,高密度脂蛋白饮食组表现出空腹糖耐量受损。高密度脂蛋白饮食20周后,高密度脂蛋白饮食组小鼠进入糖尿病状态,表现出糖耐量受损和胰岛素抵抗、胰岛体积缩小和空泡变性;与NC组相比,高密度脂蛋白饮食组肝细胞中出现大量脂滴,肝组织中AMPK磷酸化水平明显升高;与NC组相比,HFD组小鼠胸主动脉出现内皮依赖性舒张功能障碍;与NC组相比,HFD组小鼠尿蛋白水平明显升高。这些结果表明,单用高密度脂蛋白饮食诱导 20 周,就能成功建立 T2DM 小鼠模型。该模型具有胰岛素抵抗、脂肪肝、高脂血症、血管功能障碍、肾功能障碍以及胰岛和肝细胞病理变化等特征,与 T2DM 患者相似。因此,它可作为研究 T2DM 的理想动物模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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