Antiurolithiatic effect of triptonide in ethylene glycol-induced urolithiasis in rats.

IF 3.2 4区医学 Q1 Pharmacology, Toxicology and Pharmaceutics

Toxicology Mechanisms and Methods Pub Date : 2024-10-01 Epub Date: 2024-06-26 DOI:10.1080/15376516.2024.2364882

Qiang Wang, Jinghong Zhang, Xiaosong Yin, Tongwei Liu, Chuangui Li, Haibo Yuan, Ding Li

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Abstract

Urolithiasis is one of the most prevalent benign urological disorders globally with a high incidence rate. Male Sprague-Dawley rats were chemically induced to have urolithiasis and treated with triptonide and the standard antiurolithic drug cystone. Kidney weight was measured to detect calculi formation, and urinary parameters such as pH, 24-h urine volume, and protein content were measured to analyze the urolithiasis induction in rats. The inorganic ions, organic solutes, antioxidant levels, and inflammatory cytokines were measured in the experimental rats. Triptonide treatment significantly modulated the urinary pH, decreased the protein concentration, and increased the urinary outflow in urolithiasis induced rats. It also significantly decreased the urinary excretion of calcium and phosphorous and increased the excretion of magnesium, potassium, sodium, creatinine, and uric acid. SOD, CAT, and GPx levels were increased in triptonide-treated rats, and it significantly reduced the MDA levels. Triptonide treatment also decreased the levels of inflammatory cytokines and prevented the renal tissue from inflammation. To conclude, our results prove that triptonide significantly prevents calculi formation and protects renal tissue from urolithiasis-induced damage in rats. Further studies may prove triptonide a potent alternative to currently available antiurolithic drugs.

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三氪对乙二醇诱导的大鼠尿路结石症的抗尿路结石作用

尿石症是全球最常见的良性泌尿系统疾病之一，发病率很高。用化学方法诱导雄性 Sprague-Dawley 大鼠患上泌尿系结石病，并用三苯氧胺和标准抗泌尿系结石药物胱氨酸进行治疗。测量肾脏重量以检测结石的形成，并测量尿液参数，如 pH 值、24 小时尿量和蛋白质含量，以分析大鼠的尿路结石诱导情况。实验还测量了大鼠体内的无机离子、有机溶质、抗氧化剂水平和炎症细胞因子。经曲普尼治疗后，尿石症诱导大鼠的尿液 pH 值明显下降，蛋白质浓度明显降低，尿液流出量明显增加。它还能明显减少尿液中钙和磷的排泄，增加镁、钾、钠、肌酐和尿酸的排泄。经曲普尼处理的大鼠体内的 SOD、CAT 和 GPx 含量增加，MDA 含量明显降低。三苯氧胺还能降低炎症细胞因子的水平，防止肾组织发炎。总之，我们的研究结果证明，曲普奈德能明显阻止结石的形成，保护大鼠肾组织免受尿石症引起的损伤。进一步的研究可能会证明三肽类药物是目前可用的抗尿路结石药物的有效替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods 医学-毒理学

CiteScore

6.60

自引率

3.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.