HGF, HNRPD, and sFLT1 Interfere with the Induction of VEGF in Patients with Severe Psoriasis.

IF 2.2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Current molecular medicine Pub Date : 2024-06-24 DOI:10.2174/0115665240307495240605101526

Vladimir Sobolev, Madina Muminova, Olga Zhukova, Elena Denisova, Anna Soboleva, Nikolay Potekaev, Irina Korsunskaya, Alexandre Mezentsev

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引用次数: 0

Abstract

Background: Restructuring of dermal microcapillaries is one of the hallmarks of plaque psoriasis. To control the proliferation of vascular endothelial cells, vascular endothelial growth factor (VEGF) promotes the remodeling of the existing blood vessels and angiogenesis.

Objective: This study aimed to explain the lowering protein and mRNA levels of VEGF in lesional skin of patients with severe psoriasis (the Psoriasis Area and Severity Index, PASI > 25).

Methods: Using the method of qPCR, we assessed the expression of VEGF mRNA in lesional and nonlesional psoriatic skin. Using ELISA, we also compared the levels of VEGF in skin homogenates of psoriasis patients and healthy volunteers.

Results: We found that the exacerbation of psoriasis induced VEGF on mRNA and protein levels 12 and 20 times, respectively. We also confirmed a strong correlation between VEGF and PASI score in patients with PASI < 25. In addition, we showed that several factors, namely HGF, HNRPD, and sFLT1 interfere with the biosynthesis of VEGF in skin lesions of patients with PASI > 25%.

Conclusion: Thus, using VEGF as a biomarker to monitor the disease shall be done cautiously in patients with severe psoriasis.

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HGF、HNRPD 和 sFLT1 会干扰严重银屑病患者体内血管内皮生长因子的诱导。

背景：真皮微毛细血管的重组是斑块状银屑病的特征之一。为了控制血管内皮细胞的增殖，血管内皮生长因子（VEGF）可促进现有血管的重塑和血管生成：本研究旨在解释重度银屑病患者（银屑病面积和严重程度指数 PASI > 25）皮损皮肤中血管内皮生长因子蛋白和 mRNA 水平降低的原因：方法：我们使用 qPCR 方法评估了病变和非病变银屑病皮肤中 VEGF mRNA 的表达。我们还使用 ELISA 方法比较了银屑病患者和健康志愿者皮肤匀浆中 VEGF 的水平：结果：我们发现，银屑病加重会诱导血管内皮生长因子的 mRNA 和蛋白质水平分别增加 12 倍和 20 倍。我们还证实，在 PASI 小于 25 的患者中，VEGF 与 PASI 评分之间存在很强的相关性。此外，我们还发现几个因素，即 HGF、HNRPD 和 sFLT1 会干扰 PASI > 25% 患者皮损中 VEGF 的生物合成：因此，将血管内皮生长因子作为监测重症银屑病患者病情的生物标志物应慎重使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current molecular medicine 医学-医学：研究与实验

CiteScore

5.00

自引率

4.00%

发文量

141

审稿时长

4-8 weeks

期刊介绍： Current Molecular Medicine is an interdisciplinary journal focused on providing the readership with current and comprehensive reviews/ mini-reviews, original research articles, short communications/letters and drug clinical trial studies on fundamental molecular mechanisms of disease pathogenesis, the development of molecular-diagnosis and/or novel approaches to rational treatment. The reviews should be of significant interest to basic researchers and clinical investigators in molecular medicine. Periodically the journal invites guest editors to devote an issue on a basic research area that shows promise to advance our understanding of the molecular mechanism(s) of a disease or has potential for clinical applications.