Deep integration of metabolome and transcriptome characterizes alkaloid metabolism in Houttuynia cordata

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-06-19 DOI:10.1016/j.ygeno.2024.110881

Xue Jiang , Qian Wang , Jingtian Yang , Baoguo Du , Zhaodi Yuan , Hongyi Liu , Jiayi Yuan , Yang Zhang , Liao Chen , Lei Liu

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引用次数: 0

Abstract

Alkaloids are the main medicinal components in Houttuynia cordata. In this study, two accessions 6# and 7# of H. cordata underwent thorough metabolomic analyses to identify and quantify alkaloid phytometabolites. It turned out that the alkaloid types were largely similar between 6# and 7#, and the identified 81 alkaloids could be divided into nine structural classes. However, the content of alkaloids in the two accessions was quite different. According to transcriptome data, a total of 114 differentially expressed genes related to alkaloid metabolism were screened. The alkaloid synthesis pathway of the two varieties was mainly different in the isoquinoline alkaloid biosynthesis and indole alkaloid biosynthesis; four genes A22110063c_transcript_59323, A22110063c_transcript_60118, A22110063c_transcript_51672 and A22110063c_transcript_48784 were highly expressed in 7#, which could be key candidate genes of alkaloid metabolism and warrant further analysis. These results provide a reference for the medicinal application of H. cordata and breeding alkaloid rich varieties.

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代谢组和转录组的深度整合描述了蕺菜生物碱代谢的特征。

生物碱是蕺菜的主要药用成分。本研究对蕺菜的两个登录品 6#和 7#进行了全面的代谢组学分析，以鉴定和量化生物碱类植物代谢物。结果表明，6#和 7#的生物碱类型基本相似，鉴定出的 81 种生物碱可分为 9 个结构类别。但是，两种植物中生物碱的含量却大不相同。根据转录组数据，共筛选出 114 个与生物碱代谢相关的差异表达基因。两个品种的生物碱合成途径主要在异喹啉生物碱的生物合成和吲哚生物碱的生物合成方面存在差异；A22110063c_transcript_59323、A22110063c_transcript_60118、A22110063c_transcript_51672和A22110063c_transcript_48784四个基因在7#中高表达，可能是生物碱代谢的关键候选基因，值得进一步分析。这些结果为虫草的药用和培育富含生物碱的品种提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567