Genomic and immunocyte characterisation of bloodstream infection caused by Klebsiella pneumoniae.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Wei Yu, Chen Huang, Xiang Lian, Lushun Jinag, Hong Li, Ping Shen, Yonghong Xiao
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引用次数: 0

Abstract

Objectives: The aim of this study was to evaluate the characteristics of immunocyte associated with bloodstream infection (BSI) caused by Klebsiella pneumoniae (Kpn).

Methods: Patients with BSI-Kpn were included from 2015 to 2022 in our hospital. Immunocyte subpopulations of enrolled BSI-Kpn patients were tested on the same day of blood culture using multicolor flow cytometry analysis. Antibiotic susceptibility test was determined by agar dilution or broth dilution method. All included isolates were subjected to whole genome sequencing and comparative genomics analysis. Clinical and genetic data were integrated to investigate the risk factors associated with clinical outcome.

Results: There were 173 patients with non-duplicate BSI-Kpn, including 81 carbapenem-resistant Kpn (CRKP), 30 extended-spectrum β-lactamases producing Kpn (ESBL-Kpn), 62 none CRKP or ESBL-Kpn (S-Kpn). Among 68 ST11-CRKP isolates, ST11-O2v1:KL64 was the most common serotypes cluster (77.9%, 53/68), followed by ST11-OL101: KL47 (13.2%, 9/68). Compared with CSKP group, subpopulations of immunocyte in patients with CRKP were significantly lower (P < 0.01). In patients with ST11-O2v1:KL64 BSI-Kpn, the level of cytotoxic T lymphocytes (CD3 + CD8 +) is the highest, while the B lymphocytes (CD3-CD19 +) was the least. In addition, the level of immunocyte in patients with Kpn co-harbored clpV-ybtQ-qacE were lower than that in patients with Kpn harbored one of clpV, ybtQ or qacE and without these three genes. Furthermore, co-existence of clpV-ybtQ-qacE was independently associated with a higher risk for 30-day mortality.

Conclusions: The results demonstrate that patients with BSI-CRKP, especially for ST11-O2v1:KL64, exhibit lower leukomonocyte counts. In addition, BSI-Kpn co-harbored clpV-ybtQ-qacE is correlated to higher 30-day mortality.

肺炎克雷伯氏菌引起的血流感染的基因组和免疫细胞特征。
研究目的本研究旨在评估肺炎克雷伯菌(Kpn)引起的血流感染(BSI)相关免疫细胞的特征:方法:纳入我院2015年至2022年的BSI-Kpn患者。采用多色流式细胞术分析方法,在血液培养的当天对入选的 BSI-Kpn 患者的免疫细胞亚群进行检测。抗生素敏感性检测采用琼脂稀释法或肉汤稀释法。所有纳入的分离株都进行了全基因组测序和比较基因组学分析。整合临床和基因数据,研究与临床结果相关的风险因素:结果:共有173例非重复BSI-Kpn患者,其中包括81例碳青霉烯耐药Kpn(CRKP)、30例产扩谱β-内酰胺酶Kpn(ESBL-Kpn)、62例无CRKP或ESBL-Kpn(S-Kpn)。在 68 个 ST11-CRKP 分离物中,ST11-O2v1:KL64 是最常见的血清型群(77.9%,53/68),其次是 ST11-OL101:KL47(13.2%,9/68)。与 CSKP 组相比,CRKP 患者的免疫细胞亚群明显降低(P 结论:CRKP 患者的免疫细胞亚群明显低于 CSKP 组:结果表明,BSI-CRKP 患者,尤其是 ST11-O2v1:KL64 患者,白细胞计数较低。此外,BSI-Kpn 共载 clpV-ybtQ-qacE 与较高的 30 天死亡率相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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