Frequencies of micronuclei in buccal cells and their spatial distribution in a population living in proximity to coal mining areas in southern Brazil

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-05-31 DOI:10.1016/j.mrgentox.2024.503783

Niely Galeão da Rosa Moraes , Alicia da Silva Bonifácio , Fernanda Oliveira Reis , Thais dos Anjos Velho , Paula Florencio Ramires , Rodrigo de Lima Brum , Julia Oliveira Penteado , Flávio Manoel Rodrigues Da Silva Júnior

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Abstract

The extraction and burning of coal release genotoxic pollutants, and understanding the relationship between genetic damage and the spatial distribution of residences in coal-using regions is crucial. The study aimed to conduct a spatial analysis of genotoxic damage through the of micronuclei (MNs) number and their proximity to coal mining/burning in the largest coal exploration region in Brazil. In this study, the detection of genotoxic damage was performed using the MN assay in oral cells of residents exposed to coal mining activities. Spatial analysis was conducted using QGIS 3.28.10 based on information obtained from a questionnaire administered to the population. Multiple linear regression analysis was carried out to assess the influence of the distance from residential areas to polluting sources on the number of MNs found. Additionally, Spearman's correlation was performed to identify the strength and direction of the association between the frequency of MNs and each of the polluting sources. A total of 147 MNs were quantified among all participants in the coal mining region. Notably, residents living within 2 km and 10 km of pollution sources exhibited the highest prevalence of MNs. The analysis demonstrated a significant correlation between closer proximity to pollution sources and increased MN frequency, underscoring the spatial relationship between these sources and genotoxic damage. Environmental pollutants from anthropogenic sources present a major health risk, potentially leading to irreversible damage. The spatial analysis in this study highlights the importance of targeted public policies. These policies should aim for a sustainable balance between economic development and public health, promoting effective measures to mitigate environmental impacts and protect community health.

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巴西南部煤矿开采区附近居民口腔细胞中的微核频率及其空间分布情况

煤炭的开采和燃烧会释放基因毒性污染物，因此了解基因损伤与用煤地区居民点空间分布之间的关系至关重要。这项研究旨在通过微核（MNs）数量及其与巴西最大煤炭开采/燃烧地区的距离，对遗传毒性损害进行空间分析。在这项研究中，使用微核检测法对暴露于煤矿开采活动的居民的口腔细胞进行了基因毒性损伤检测。根据从居民调查问卷中获得的信息，使用 QGIS 3.28.10 进行了空间分析。进行了多元线性回归分析，以评估居民区与污染源的距离对发现的 MN 数量的影响。此外，还进行了斯皮尔曼相关分析，以确定流纹态氮化物出现频率与各污染源之间的关联强度和方向。在煤矿开采区的所有参与者中，共量化了 147 个 MNs。值得注意的是，居住在污染源 2 公里和 10 公里范围内的居民表现出最高的多发性喉炎发病率。分析表明，距离污染源越近，出现 MN 的频率越高，两者之间存在明显的相关性，这突出表明了污染源与遗传毒性损害之间的空间关系。人为来源的环境污染物对健康构成重大威胁，可能导致不可逆转的损害。本研究的空间分析强调了有针对性的公共政策的重要性。这些政策的目标应该是在经济发展和公众健康之间实现可持续的平衡，促进采取有效措施来减轻环境影响和保护社区健康。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.