Chitosan-based endodontic irrigation solutions and TGF-β1 treatment: Creating the most favourable environment for the survival and proliferation of stem cells of the apical papilla in vitro

IF 5.4 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2024-06-18 DOI:10.1111/iej.14112

Roumaissa Belkadi, Diana Sanz-Serrano, Francesc Ventura, Montse Mercade

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引用次数: 0

Abstract

Background

The dental pulp's environment is essential for the regulation of mesenchymal stem cells' homeostasis and thus, it is of great importance to evaluate the materials used in regenerative procedures.

Aim

To assess in vitro (i) the effect of chitosan nanoparticles, 0.2% chitosan irrigation solution, Dual Rinse®, 17% EDTA, 10% citric acid and 2.5% NaOCl on DSCS viability; (ii) the effect of different concentrations of TGF-β1 on DCSC proliferation; and (iii) whether treatment with TGF-β1 following exposure to the different irrigation solutions could compensate for their negative effects.

Methodology

(i) DSCS were treated with three dilutions (1:10, 1:100 and 1:1000) of the six irrigation solutions prepared in DMEM for 10 and 60 min to assess the effect on viability. (ii) The effect of different concentrations (0, 1, 5 and 10 ng/mL) of TGF-β1 on DCSC proliferation was assessed at 1, 3 and 7 days. (iii) The proliferative effect of TGF-β1 following 10-min exposure to 1:10 dilution of each irrigation solution was also tested. We used MTT assay to assess viability and proliferation. We performed statistical analysis using Prism software.

Results

(i) The different endodontic irrigation solutions tested showed a significant effect on cell viability (p ≤ .0001). Significant interactions between the endodontic irrigation solutions and their dilutions were also found for all parameters (p ≤ .0001). Chitosan nanoparticles and 0.2% chitosan irrigation solution were the least cytotoxic to DSCS whilst 2.5% NaOCl was the most cytotoxic followed by 17% EDTA. (ii) TGF-β1 at concentrations of 1 and 5 ng/mL resulted in significantly higher proliferation compared to the control group. (iii) Exposure to 17% EDTA or 2.5% NaOCl for 10 min was sufficient to make DSCS cells refractory to the proliferative effects of TGF-β1. DSCS groups treated with TGF-β1 following exposure to chitosan nanoparticles, 0.2% chitosan irrigation solution, Dual Rinse® and 10% CA demonstrated significantly higher proliferation compared to non-TGF-β1-treated groups (p ≤ .0001, p ≤ .0001, p ≤ .0001 and p = .01 respectively).

Conclusions

The current study offers data that can be implemented to improve the outcome of regenerative endodontic procedures by using less toxic irrigation solutions and adding TGF-β1 to the treatment protocol.

Abstract Image

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基于壳聚糖的牙髓冲洗液和 TGF-β1 治疗：为根尖乳头干细胞的体外生存和增殖创造最有利的环境。

背景：目的：在体外评估(i)壳聚糖纳米颗粒、0.2%壳聚糖灌洗液、Dual Rinse®、17% EDTA、10%柠檬酸和2.方法：(i) 用 DMEM 制备的六种灌洗液的三种稀释液（1:10、1:100 和 1:1000）处理 DSCS 10 分钟和 60 分钟，以评估其对活力的影响。(ii) 不同浓度（0、1、5 和 10 ng/mL）的 TGF-β1 对 DCSC 增殖的影响分别在 1、3 和 7 天进行评估。(iii) 每种灌流液 1:10 稀释 10 分钟后，还测试了 TGF-β1 的增殖效应。我们使用 MTT 法评估活力和增殖。结果：(i) 测试的不同牙髓冲洗液对细胞活力有显著影响（p ≤ .0001）。牙髓冲洗液及其稀释液之间的相互作用对所有参数也有显著影响（p ≤ .0001）。壳聚糖纳米颗粒和 0.2% 壳聚糖冲洗液对 DSCS 的细胞毒性最小，而 2.5% NaOCl 的细胞毒性最大，其次是 17% EDTA。(ii) 与对照组相比，浓度为 1 和 5 ng/mL 的 TGF-β1 导致增殖明显增加。(iii) 暴露于 17% EDTA 或 2.5% NaOCl 10 分钟足以使 DSCS 细胞对 TGF-β1 的增殖效应产生耐受性。经壳聚糖纳米颗粒、0.2% 壳聚糖灌洗液、Dual Rinse® 和 10% CA 处理的 DSCS 组与未经 TGF-β1 处理的 DSCS 组相比，增殖率明显更高（分别为 p ≤ .0001、p ≤ .0001、p ≤ .0001 和 p = .01）：本研究提供的数据可通过使用毒性较低的冲洗液和在治疗方案中添加 TGF-β1 来改善牙髓再生术的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.