Occurrence of carbapenem-resistant hypervirulent Klebsiella pneumoniae in oysters in Egypt: a significant public health issue.

IF 4.6 2区医学 Q1 MICROBIOLOGY

Annals of Clinical Microbiology and Antimicrobials Pub Date : 2024-06-17 DOI:10.1186/s12941-024-00711-5

Rahma Mohammed, Sara M Nader, Dalia A Hamza, Maha A Sabry

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引用次数: 0

Abstract

Background: The global dissemination of critical-priority carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) via food sources represents a significant public health concern. Epidemiological data on CR-hvKp in oysters in Egypt is limited. This study aimed to investigate the potential role of oysters sold in Egypt as a source for carbapenem-resistant K. pneumoniae (CRKP), hypervirulent K. pneumoniae (hvKp), and CR-hvKp and assess associated zoonotic risks.

Methods: A sample of 330 fresh oysters was randomly purchased from various retail fish markets in Egypt and divided into 33 pools. Bacteriological examination and the identification of Klebsiella pneumoniae were performed. Carbapenem resistance in K. pneumoniae isolates was determined by phenotypic and molecular methods. Additionally, the presence of hypervirulent K. pneumoniae was identified based on virulence gene markers (peg-344, rmpA, rmpA2, iucA, and iroB), followed by a string test. The clustering of CR-hvKp strains was carried out using R with the pheatmap package.

Results: The overall prevalence of K. pneumoniae was 48.5% (16 out of 33), with 13 isolates displaying carbapenem resistance, one intermediate resistance, and two sensitive. Both carbapenem-resistant K. pneumoniae and carbapenem-intermediate-resistant K. pneumoniae strains exhibited carbapenemase production, predominantly linked to the bla_VIM gene (68.8%). HvKp strains were identified at a rate of 62.5% (10/16); notably, peg-344 was the most prevalent gene. Significantly, 10 of the 13 CRKP isolates possessed hypervirulence genes, contributing to the emergence of CR-hvKp. Moreover, cluster analysis revealed the clustering of two CR-hvKp isolates from the same retail fish market.

Conclusion: This study provides the first insight into the emergence of CR-hvKp among oysters in Egypt. It underscores the potential role of oysters as a source for disseminating CR-hvKp within aquatic ecosystems, presenting a possible threat to public health.

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埃及牡蛎中出现耐碳青霉烯类的高病毒性肺炎克雷伯氏菌：一个重大的公共卫生问题。

背景：耐碳青霉烯类抗生素的高病毒性肺炎克雷伯氏菌（CR-hvKp）通过食物来源在全球传播是一个重大的公共卫生问题。有关埃及牡蛎中 CR-hvKp 的流行病学数据十分有限。本研究旨在调查在埃及销售的牡蛎作为耐碳青霉烯类肺炎双球菌（CRKP）、高病毒性肺炎双球菌（hvKp）和 CR-hvKp 的来源的潜在作用，并评估相关的人畜共患病风险：方法：从埃及各零售鱼市场随机购买 330 个新鲜牡蛎样本，分成 33 个池子。进行了细菌学检查和肺炎克雷伯氏菌的鉴定。通过表型和分子方法确定了肺炎克雷伯菌分离物的碳青霉烯耐药性。此外，还根据毒力基因标记（peg-344、rmpA、rmpA2、iucA 和 iroB）以及串联试验确定了肺炎克雷伯菌是否存在高病毒性。使用 R 和 pheatmap 软件包对 CR-hvKp 菌株进行了聚类：肺炎克雷伯菌的总发病率为 48.5%（33 株中有 16 株），其中 13 株对碳青霉烯类耐药，1 株为中间耐药，2 株为敏感。耐碳青霉烯类药物的肺炎克氏菌和耐碳青霉烯类药物的中度耐药肺炎克氏菌菌株都能产生碳青霉烯酶，主要与 blaVIM 基因有关（68.8%）。HvKp 菌株的鉴定率为 62.5%（10/16）；值得注意的是，peg-344 是最常见的基因。值得注意的是，13 株 CRKP 分离物中有 10 株具有高致病力基因，这也是 CR-hvKp 出现的原因之一。此外，聚类分析显示，来自同一零售鱼市场的两个 CR-hvKp 分离物聚集在一起：本研究首次揭示了 CR-hvKp 在埃及牡蛎中的出现。它强调了牡蛎作为 CR-hvKp 在水生生态系统中传播源的潜在作用，可能对公众健康构成威胁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Microbiology and Antimicrobials MICROBIOLOGY-

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.