The pentatricopeptide repeat protein TCD6 functions RNA editing and cleavage of ndhA and is required for chloroplast development in early rice seedlings

Crop Design Pub Date : 2024-06-11 DOI:10.1016/j.cropd.2024.100063

Yunguang Sun , Licheng Kuang , Jinglin Wang , Mengshuang Gu , Yu Chen , Xiaobiao Pan , Dongzhi Lin , Yanjun Dong

{"title":"The pentatricopeptide repeat protein TCD6 functions RNA editing and cleavage of ndhA and is required for chloroplast development in early rice seedlings","authors":"Yunguang Sun , Licheng Kuang , Jinglin Wang , Mengshuang Gu , Yu Chen , Xiaobiao Pan , Dongzhi Lin , Yanjun Dong","doi":"10.1016/j.cropd.2024.100063","DOIUrl":null,"url":null,"abstract":"<div>Pentatricopeptide repeat (PPR) proteins compose one of the largest protein families in higher plants and play a role in regulating organellar gene expression. In this study, we discovered that a new rice mutant tcd6 exhibited albino phenotype and aberrant chloroplast before the three-leaf (autotrophic) seedling stage. Through Map-based cloning and complementation tests, it was shown that TCD6 encodes a chloroplast-located PPR protein, with 14 PPR motifs and an atypical DYW-like motif. In addition, the disruption of TCD6 hindered the nuclear-encoded polymerase (NEP)-dependent transcript levels for plastid genes and led to defects in the cleavage and editing of ndhA (encoding NDH subunit) in early tcd6 mutant seedlings. Taken together, our results indicate that TCD6 is indispensable for chloroplast development and involves in RNA editing and cleavage of ndhA during early seedling (autotrophic) growth of rice.</div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 3","pages":"Article 100063"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899424000120/pdfft?md5=cd0814ee0897a0f12002e6c622df607b&pid=1-s2.0-S2772899424000120-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Design","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772899424000120","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Pentatricopeptide repeat (PPR) proteins compose one of the largest protein families in higher plants and play a role in regulating organellar gene expression. In this study, we discovered that a new rice mutant tcd6 exhibited albino phenotype and aberrant chloroplast before the three-leaf (autotrophic) seedling stage. Through Map-based cloning and complementation tests, it was shown that TCD6 encodes a chloroplast-located PPR protein, with 14 PPR motifs and an atypical DYW-like motif. In addition, the disruption of TCD6 hindered the nuclear-encoded polymerase (NEP)-dependent transcript levels for plastid genes and led to defects in the cleavage and editing of ndhA (encoding NDH subunit) in early tcd6 mutant seedlings. Taken together, our results indicate that TCD6 is indispensable for chloroplast development and involves in RNA editing and cleavage of ndhA during early seedling (autotrophic) growth of rice.

查看原文本刊更多论文

五叉肽重复蛋白 TCD6 具有 RNA 编辑和切割ndhA 的功能，是早稻幼苗叶绿体发育的必需物质

五肽重复蛋白（PPR）是高等植物中最大的蛋白家族之一，在调控细胞器基因表达方面发挥作用。本研究发现，一种新的水稻突变体 tcd6 在三叶（自养）幼苗期之前表现出白化表型和叶绿体异常。通过基于图谱的克隆和互补试验表明，TCD6编码一种叶绿体定位的PPR蛋白，具有14个PPR基序和一个非典型的DYW样基序。此外，TCD6的破坏阻碍了核编码聚合酶（NEP）依赖的质体基因转录本水平，并导致早期ttcd6突变体幼苗中ndhA（编码NDH亚基）的裂解和编辑缺陷。综上所述，我们的研究结果表明，TCD6 对叶绿体的发育是不可或缺的，它参与了水稻早期幼苗（自养）生长过程中的 RNA 编辑和 ndhA 的裂解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Crop Design

自引率

0.00%

发文量