Functional insights of digestion, absorption, and immunity in different segments of the intestine in Hemibarbus labeo from transcriptomic analysis

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Yaoping Lv , Xinming Gao , Qingmin Dai , Ling Zhu , Siqi Liu , Zehui Hu , Junkai Lu , Haidong Zhou , Jing Jin , Zufei Mei
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Abstract

The intestine is an important organ for food digestion and absorption and body immunity in fish. In this study, we investigated the abundance of transcripts from different segments of the intestinal tract using transcriptome sequencing technology in Hemibarbus labeo, to provide functional insights into digestion, absorption, and immunity in the anterior intestine (AI), middle intestine (MI), and posterior intestine (PI). We found 5646 differentially expressed genes (DEGs), which were significantly enriched to GO terms of carbohydrate metabolic process, transmembrane transport, iron ion binding, lipid metabolic process, and KEGG pathway of fat digestion and absorption, mineral absorption, protein digestion and absorption, vitamin digestion and absorption, indicating that the digestion and absorption function of food is different in AI, MI, and PI. In practice, most genes, enriched in the KEGG pathway for digestion and absorption of nutrients, are upregulated in AI and MI, indicating stronger roles for food digestion and absorption in these segments. Furthermore, we found that genes involved in the KEGG pathway of lysosome and endocytosis pathway are upregulated in PI, suggesting stronger antigen-presenting capabilities in PI. However, some cytokine receptor genes, including ccr4, cxcr2, tnfrsf9, il6r, csf3r, and cxcr4, are highly expressed in AI, reflecting the regional immune specialization in different segments. This study provides functional insights into digestion, absorption, and immunity in different segments of the intestine and supports the regional functional specialization within different segments of the intestine in H. labeo.

Abstract Image

通过转录组分析了解拉贝虫不同肠段的消化、吸收和免疫功能
肠道是鱼类消化吸收食物和机体免疫的重要器官。本研究利用转录组测序技术,研究了拉比目鱼(Hemibarbus labeo)肠道不同区段的转录本丰度,以深入了解前肠(AI)、中肠(MI)和后肠(PI)的消化、吸收和免疫功能。我们发现了5646个差异表达基因(DEGs),这些基因显著富集于碳水化合物代谢过程、跨膜运输、铁离子结合、脂质代谢过程等GO术语,以及脂肪消化吸收、矿物质吸收、蛋白质消化吸收、维生素消化吸收等KEGG通路,表明食物的消化吸收功能在前肠、中肠和后肠中是不同的。实际上,富集在营养物质消化吸收 KEGG 通路中的大多数基因在 AI 和 MI 中上调,这表明食物消化吸收在这些区段中的作用更强。此外,我们还发现,参与溶酶体和内吞途径 KEGG 通路的基因在胰岛细胞中上调,这表明胰岛细胞具有更强的抗原递呈能力。然而,一些细胞因子受体基因,包括 ccr4、cxcr2、tnfrsf9、il6r、bsf3r 和 cxcr4 在 AI 中高表达,反映了不同节段的区域免疫特化。这项研究提供了关于不同肠段消化、吸收和免疫的功能性见解,并支持了拉贝休不同肠段的区域功能特化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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