Effect of an endothelial regulatory module on plasma proteomics in exercising horses

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Mahmoud Amiri Roudbar , Maria K. Rosengren , Seyedeh Fatemeh Mousavi , Kim Fegraeus , Rakan Naboulsi , Jennifer R.S. Meadows , Gabriella Lindgren
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Abstract

Elite performing exercise requires an intricate modulation of the blood pressure to support the working muscles with oxygen. We have previously identified a genomic regulatory module that associates with differences in blood pressures of importance for elite performance in racehorses. This study aimed to determine the effect of the regulatory module on the protein repertoire. We sampled plasma from 12 Coldblooded trotters divided into two endothelial regulatory module haplotype groups, a sub-elite performing haplotype (SPH) and an elite performing haplotype (EPH), each at rest and exercise. The haplotype groups and their interaction were interrogated in two analyses, i) individual paired ratio analysis for identifying differentially abundant proteins of exercise (DAPE) and interaction (DAPI) between haplotype and exercise, and ii) unpaired ratio analysis for identifying differentially abundant protein of haplotype (DAPH). The proteomics analyses revealed a widespread change in plasma protein content during exercise, with a decreased tendency in protein abundance that is mainly related to lung function, tissue fluids, metabolism, calcium ion pathway and cellular energy metabolism. Furthermore, we provide the first investigation of the proteome variation due to the interaction between exercise and related blood pressure haplotypes, which this difference was related to a faster switch to the lipoprotein and lipid metabolism during exercise for EPH. The molecular signatures identified in the present study contribute to an improved understanding of exercise-related blood pressure regulation.

Abstract Image

内皮调节模块对运动马血浆蛋白质组学的影响
精英运动需要对血压进行复杂的调节,以便为工作肌肉提供氧气。我们之前已经确定了一个基因组调控模块,该模块与对赛马精英表现至关重要的血压差异有关。本研究旨在确定该调控模块对蛋白质复合物的影响。我们采集了 12 匹冷血赛马的血浆样本,将其分为两个内皮调控模块单倍型组,一个是亚精英赛马单倍型(SPH),另一个是精英赛马单倍型(EPH)。对单倍型组及其相互作用进行了两项分析:i)单个配对比率分析,以确定单倍型与运动之间的运动差异丰富蛋白(DAPE)和相互作用(DAPI);ii)非配对比率分析,以确定单倍型差异丰富蛋白(DAPH)。蛋白质组学分析表明,运动时血浆蛋白质含量发生了广泛变化,蛋白质丰度呈下降趋势,这主要与肺功能、组织液、新陈代谢、钙离子通道和细胞能量代谢有关。此外,我们还首次研究了运动与相关血压单倍型之间相互作用导致的蛋白质组变化,这种差异与 EPH 在运动过程中更快地转向脂蛋白和脂质代谢有关。本研究发现的分子特征有助于更好地理解与运动相关的血压调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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