Adil Elrayah, Ke Duan, Xiong Lu, Xiaobo Lu, Jie Weng
{"title":"Calcium Phosphate Coatings with Controlled Micro/Nano-Structures for Endothelial Cells Viability","authors":"Adil Elrayah, Ke Duan, Xiong Lu, Xiaobo Lu, Jie Weng","doi":"10.51173/jt.v6i2.2545","DOIUrl":null,"url":null,"abstract":"Hydroxyapatite (HA) scaffolds produced by the accumulation of HA fibers were separately treated hydrothermally in three calcium phosphate solutions to form coatings of different micro/nano-structures. Different micro/nano-structure and morphologies have been regulated on the surface of treated HA scaffolds. Plate-like compromise flower-like morphology was obtained with solution 1 (Ca-sufficient) i.e., ratio: Ca/Ca=1%; Ca/P=1.67. Full coatings (flower-like) morphology treated after Cu-doped coating solution 2 (Cu/(Cu+Ca) = 5%; ratio: (Cu+Ca)/P = 1.67). Furthermore, partial coatings (flower-like) morphology fabricated with solution 3 (Ca-deficient and Cu-replacement), i.e., ratio: Ca/Ca=0.95%; Ca/P=1.58. The results showed the effect of hydrothermal coatings on HA scaffolds. Cultured human endothelial cells spread and proliferated better on the treated HA scaffolds than on the uncoated scaffolds, suggesting a potential effect of calcium phosphate surface morphology on endothelial cell response. Thus, it can provide an appropriate micro/nano-structure approach supporting angiogenesis capacity, which is a necessity to accelerate the time of bone healing and regeneration.","PeriodicalId":516635,"journal":{"name":"Journal of Techniques","volume":"11 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Techniques","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.51173/jt.v6i2.2545","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Hydroxyapatite (HA) scaffolds produced by the accumulation of HA fibers were separately treated hydrothermally in three calcium phosphate solutions to form coatings of different micro/nano-structures. Different micro/nano-structure and morphologies have been regulated on the surface of treated HA scaffolds. Plate-like compromise flower-like morphology was obtained with solution 1 (Ca-sufficient) i.e., ratio: Ca/Ca=1%; Ca/P=1.67. Full coatings (flower-like) morphology treated after Cu-doped coating solution 2 (Cu/(Cu+Ca) = 5%; ratio: (Cu+Ca)/P = 1.67). Furthermore, partial coatings (flower-like) morphology fabricated with solution 3 (Ca-deficient and Cu-replacement), i.e., ratio: Ca/Ca=0.95%; Ca/P=1.58. The results showed the effect of hydrothermal coatings on HA scaffolds. Cultured human endothelial cells spread and proliferated better on the treated HA scaffolds than on the uncoated scaffolds, suggesting a potential effect of calcium phosphate surface morphology on endothelial cell response. Thus, it can provide an appropriate micro/nano-structure approach supporting angiogenesis capacity, which is a necessity to accelerate the time of bone healing and regeneration.
在三种磷酸钙溶液中分别对堆积 HA 纤维生成的羟基磷灰石(HA)支架进行水热处理,以形成不同微/纳米结构的涂层。经过处理的 HA 支架表面呈现出不同的微/纳米结构和形态。使用溶液 1(钙充足),即比率为Ca/Ca=1%;Ca/P=1.67。掺铜涂层溶液 2(Cu/(Cu+Ca) = 5%;比率:(Cu+Ca)/P = 1.67)处理后可获得全涂层(花朵状)形态。此外,用溶液 3(缺钙和铜置换)制造的部分涂层(花朵状)形态,即比率:(Cu+Ca)/P = 1.67:Ca/Ca=0.95%;Ca/P=1.58。结果显示了水热涂层对 HA 支架的影响。培养的人内皮细胞在经过处理的 HA 支架上的扩散和增殖效果优于未涂层的支架,这表明磷酸钙表面形态对内皮细胞反应有潜在影响。因此,它可以提供一种支持血管生成能力的适当微/纳米结构方法,而血管生成能力是加速骨愈合和再生的必要条件。