The Effect of Different Ethanol Concentrations as The Extraction Solvent on Total Flavonoid Content of White Mulberry Leaves Extracts (Morus alba L.)

Harpolia Cartika, Khairun Nida, Ilma Atania Ahya
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Abstract

White mulberry leaves (Morus alba L.) contain flavonoid which empirically have many health benefits, such as reducing fever, relieving headaches and throats, coughs, dysentery, digestive disorders, flu, and according to research it has antioxidant activity. Many factors effect the total flavonoid content, one of which is the concentration of the extraction solvent. The purpose of the research was to determine the effect of different ethanol concentrations as the extraction solvent on total flavonoid content of white mulberry (Morus alba L.) leaves extract. White mulberry leaves (Morus alba L.) were extracted using the maceration method, the extract was tested for phytochemical screening and thin layer chromatography using chloroform: ethyl acetate (6: 4) as the eluent. The resulting spots were observed visually and with λ 254 nm and λ 366 nm UV light. Determination of flavonoid content by UV-Vis spectrophotometry using the AlCl3 colorimetric method at a maximum wavelength of 415 nm. The research results showed that the yield of 50% and 96% ethanol extract of white mulberry leaves (Morus alba L.) was 17.42 % and 11.04 %. Phytochemical screening showed that both extracts positively contained flavonoids. The presence of flavonoids was confirmed using a thin layer chromatography test, resulting in an average Rf value for 50% and 96% ethanol extracts are 0.71 and 0.70, close to quercetin Rf standard is 0,73 as comparison. The total flavonoid content obtained from the 50% ethanol extract was 36.0902 mgQE/g and the 96% ethanol extract was 56.4699 mgQE/g. The conclusions are the concentration of the extraction solvent effect the yield and total flavonoid content.
不同浓度乙醇作为萃取溶剂对白桑叶提取物总黄酮含量的影响
白桑叶(Morus alba L.)含有黄酮类化合物,根据经验,它对健康有许多益处,如退烧、缓解头痛、喉咙痛、咳嗽、痢疾、消化系统疾病、流感等,根据研究,它还具有抗氧化活性。影响总黄酮含量的因素很多,其中之一是提取溶剂的浓度。本研究的目的是确定不同浓度的乙醇作为提取溶剂对白桑叶(Morus alba L.)提取物中总黄酮含量的影响。白桑叶(Morus alba L.)采用浸渍法提取,提取物经植物化学筛选和薄层色谱法检测,以氯仿:乙酸乙酯(6:4)为洗脱剂。用肉眼和 λ 254 nm 和 λ 366 nm 紫外光观察所产生的斑点。采用 AlCl3 比色法,在最大波长 415 nm 处用紫外可见分光光度法测定黄酮类化合物的含量。研究结果表明,白桑叶(Morus alba L.)50% 和 96% 乙醇提取物的产量分别为 17.42 % 和 11.04 %。植物化学筛选表明,两种提取物都含有黄酮类化合物。黄酮类化合物的存在是通过薄层色谱测试确认的,结果 50%和 96%乙醇提取物的平均 Rf 值分别为 0.71 和 0.70,接近槲皮素标准 Rf 值 0.73。50%乙醇提取物的总黄酮含量为 36.0902 mgQE/g,96%乙醇提取物的总黄酮含量为 56.4699 mgQE/g。结论是萃取溶剂的浓度会影响产量和总黄酮含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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