CopG1, a Novel Transcriptional Regulator Affecting Symbiosis in Bradyrhizobium sp. SUTN9-2

Biology Pub Date : 2024-06-05 DOI:10.3390/biology13060415

Praneet Wangthaisong, P. Piromyou, Pongpan Songwattana, Tarnee Phimphong, Apisit Songsaeng, Natcha Pruksametanan, Pakpoom Boonchuen, Jenjira Wongdee, K. Teamtaisong, N. Boonkerd, Shusei Sato, P. Tittabutr, N. Teaumroong

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Abstract

The symbiotic interaction between leguminous and Bradyrhizobium sp. SUTN9-2 mainly relies on the nodulation process through Nod factors (NFs), while the type IV secretion system (T4SS) acts as an alternative pathway in this symbiosis. Two copies of T4SS (T4SS1 and T4SS2) are located on the chromosome of SUTN9-2. ΔT4SS1 reduces both nodule number and nitrogenase activity in all SUTN9-2 nodulating legumes. The functions of three selected genes (copG1, traG1, and virD21) within the region of T4SS1 were examined. We generated deleted mutants and tested them in Vigna radiata cv. SUT4. ΔtraG1 and ΔvirD21 exhibited lower invasion efficiency at the early stages of root infection but could be recently restored. In contrast, ΔcopG1 completely hindered nodule organogenesis and nitrogenase activity in all tested legumes. ΔcopG1 showed low expression of the nodulation gene and ttsI but exhibited high expression levels of the T4SS genes, traG1 and trbE1. The secreted proteins from ΔT4SS1 were down-regulated compared to the wild-type. Although ΔcopG1 secreted several proteins after flavonoid induction, T3SS (nopP and nopX) and the C4-dicarboxylate transporter (dct) were not detected. These results confirm the crucial role of the copG1 gene as a novel key regulator in the symbiotic relationship between SUTN9-2 and legumes.

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影响巴西根瘤菌 SUTN9-2 共生的新型转录调节器 CopG1

豆科植物与巴西根瘤菌（Bradyrhizobium sp. SUTN9-2）之间的共生作用主要依赖于通过节点因子（NFs）进行的结瘤过程，而 IV 型分泌系统（T4SS）则是这种共生作用的另一种途径。T4SS 的两个拷贝（T4SS1 和 T4SS2）位于 SUTN9-2 的染色体上。ΔT4SS1 会减少所有 SUTN9-2 有节豆科植物的结核数量和氮酶活性。我们研究了 T4SS1 区域内三个选定基因（copG1、traG1 和 virD21）的功能。我们生成了删除突变体，并在 Vigna radiata cv. SUT4 中进行了测试。ΔtraG1 和 ΔvirD21 在根部感染的早期阶段表现出较低的侵染效率，但最近可以恢复。相比之下，ΔcopG1 完全阻碍了所有受试豆科植物的结核器官发生和氮酶活性。ΔcopG1 的结核基因和 ttsI 表达量较低，但 T4SS 基因、traG1 和 trbE1 的表达量较高。与野生型相比，ΔT4SS1 的分泌蛋白下调。虽然ΔcopG1在类黄酮诱导后分泌了多种蛋白质，但没有检测到T3SS（nopP和nopX）和C4-二羧酸转运体（dct）。这些结果证实了 copG1 基因在 SUTN9-2 与豆科植物的共生关系中发挥着关键作用。

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Biology

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