Utilization of palm oil and palm oil effluent as a carbon source for the production of polyhydroxybutyrate co-polyhydroxyhexanoate P(HB-co-HHx) a biopolymer in bacillus sp

IF 2.8 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2024-06-06 DOI:10.1002/jctb.7689

Vrunda N Katagi, Roopashri N Arekal, M Somashekara Divyashree

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Abstract

BACKGROUND

Biodegradable polymers are gaining a reputation as demand is increasing globally. Amongst these Polyhydroxyalkanoates (PHA) are attracting market attention as a consequence of their properties similar to those of conventional plastics. PHA is a natural polyester that is stored as an intracellular carbon (C) energy reserve of bacteria. This biopolymer can be extracted and used in place of petrochemical plastic. Medium chain-length (mcl) PHA, specifically poly hydroxybutyrate-co-hydroxyhexanoate P (HB-co-HHx) copolymer produced by Bacillus, has gained practical attention owing to its superior physicochemical properties compared to the commonly synthesized PHB homopolymer. Co-polymer production is dependent on C sources provided in the growth medium. In the present study we have employed palm oil and palm oil effluent as C source.

RESULTS

In the current study, it was observed that a significant amount of PHA and biomass was produced when the effluent was prepared at a concentration of 100% instead of water for preparing the growth medium. The production of biomass and PHA showed a range of 4.8–8and 1.5–4 g L⁻¹, respectively. This means that the PHA yield obtained using the effluent was 50–80% of the control, whereas the control yielded only 40–50%.

CONCLUSION

Palm industry waste can be used as a substrate in bioprocessing industries. Proton nuclear magnetic resonance analysis of PHA obtained herein indicates that it consists of the mcl polymer P (HB-co-HHx). © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

Abstract Image

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利用棕榈油和棕榈油废水作为碳源，在芽孢杆菌中生产聚羟基丁酸共聚羟基己酸酯 P(HB-co-HHx)生物聚合物

随着全球对可生物降解聚合物的需求不断增加，这种聚合物的声誉也日益提高。其中，聚羟基烷酸酯（PHA）因其与传统塑料相似的特性而备受市场关注。PHA 是一种天然聚酯，储存在细菌的细胞内碳（C）能量储备中。这种生物聚合物可被提取出来，用于替代石化塑料。中链长（cl）PHA，特别是由芽孢杆菌生产的聚羟基丁酸-聚羟基己酸 P（HB-co-Hx）共聚物，由于其理化性质优于通常合成的 PHB 均聚物而受到实际关注。共聚物的生产取决于生长培养基中提供的碳源。在本研究中，我们使用了棕榈油和棕榈油污水作为 C 源。在本研究中，我们观察到，当污水浓度为 100%，而不是用水来制备生长介质时，产生了大量 PHA 和生物质。生物量和 PHA 的产量范围分别为 4.8-8 和 1.5-4 g L-1。这意味着使用该污水获得的 PHA 产量是对照组的 50-80%，而对照组的产量仅为 40-50%。此处获得的 PHA 的质子核磁共振分析表明，它由 mcl 聚合物 P（HB-co-HHx）组成。© 2024 作者。化学技术与生物技术期刊》由约翰威利父子有限公司代表化学工业学会（SCI）出版。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.